ABSTRACT: The Southeastern United States contains some of the most diverse aquatic biota in the world. Conservation planning in the region requires an assessment of the degree of fragmentation of streams and rivers and potential impacts on fish populations. With a connectivity assessment, conservation actions can be prioritized and resources efficiently applied to removing or remediating barriers and connecting fish to the habitats they require to complete their life cycles. SARP and its partners identified a need for a comprehensive dataset of fish barrier locations, attributes, and links to basic river maps. Recent efforts to meet these needs through various partnerships and funding mechanisms across the Southeast provide the [...]