Effect of SDS and Proteinase K on whole blood preserved in lysis buffer for Hawaiian Island avian malaria detection, 2005-2006
Dates
Publication Date
2020
Start Date
2005-01-01
End Date
2006-01-01
Citation
Atkinson, C.T., 2020, Hawaii Island forest bird avian malaria detection using whole blood preserved in lysis buffer, 2005-2006: U.S. Geological Survey data release, https://doi.org/10.5066/P98CVJJG.
Summary
It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set reports raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an infected Hawaii Amakihi that were preserved in PBS (phosphate buffered saline), TEN (Tris-Ethylenediaminetetraacetic Acid) Buffer, [...]
Summary
It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set reports raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an infected Hawaii Amakihi that were preserved in PBS (phosphate buffered saline), TEN (Tris-Ethylenediaminetetraacetic Acid) Buffer, or TEN buffer that was subsequently treated with SDS (sodium dodecyl sulfate - ethylenediaminetetracetic acid) and Proteinase K.
Click on title to download individual files attached to this item.
Effect of SDS and ProtK.xml Original FGDC Metadata
View
24.6 KB
application/fgdc+xml
Effect of SDS and ProtK.csv
1.93 KB
text/csv
Purpose
Recent detections of avian malarial parasites in native and non-native forest birds at Hakalau Forest National Wildlife Refuge and reports of epidemic transmission of the disease in high elevation habitats (Freed et al. 2005) as well as controversy over accuracy of the PCR diagnostic test that was being used (Jarvi et al. 2002) led to a request by U.S. Fish and Wildlife Service to see if existing blood samples that were preserved in a DNA lysis buffer could be used for independent confirmation of the findings with antibody based serological methods (Jarvi et al. 2002). The primary objective of this study was to test whether some DNA buffers used for preservation of blood samples (Feldman et al. 1995, Jarvi et al. 2002) cause denaturation and loss of antigenicity of antibody molecules. If the buffer does not destroy antigenicity of these molecules, then the samples can be used in serological assays to provide an independent assessment of the accuracy of the PCR test. REFERENCES: (1) Feldman, R. A., L. A. Freed, and R. L. Cann. 1995. A PCR test for avian malaria in Hawiian birds. Molecular Ecology 4: 663-673. (2) Freed, L. A., R. L. Cann, M. L. Goff, W. A. Kuntz, and G. R. Bodner. 2005. Increase in avian malria at upper elevation in Hawai`i. The Condor 107: 753-764. (3) Jarvi, S. I., J. J. Schultz, and C. T. Atkinson. 2002. PCR diagnostics underestimate the prevalence of avian malaria (Plasmodium relictum) in experimentally-infected passerines. Journal of Parasitology 88: 153-158.
