HPLC and ELISA analyses of larval bile acids from Pacific and western brook lampreys
Citation
S.-S. Yun et al. HPLC and ELISA analyses of larval bile acids from Pacific
and western brook lampreys. Steroids 68 (2003) 515–523
Summary
Abstract Comparative studies were performed on two native lamprey species, Pacific lamprey (Lampetra tridentata) and western brook lamprey (Lampetra richardsoni) from the Pacific coast along with sea lamprey (Petromyzon marinus) from the Great Lakes, to investigate their bile acid production and release.HPLCand ELISA analyses of the gall bladders and liver extract revealed that the major bile acid compound from Pacific and western brook larval lampreys was petromyzonol sulfate (PZS), previously identified as a migratory pheromone in larval sea lamprey. An ELISA for PZS has been developed in aworking range of 20 pg–10 ng per well. The tissue concentrations of PZS in gall bladder were 127.40, 145.86, and 276.96g/g body mass in sea lamprey, [...]
