Microsatellite genotypes and DNA yield of artificially produced larval pallid sturgeon, Scaphirhynchus albus

Publication Date

2021-03-02

Start Date

2017-12-08

End Date

2019-11-02

Flamio, R., Jr., Gocker, R.M., Buhman, A.C., Heist, E.J., Chojnacki, K.A., and Delonay, A.J., 2021, Microsatellite genotypes and DNA yield of artificially produced larval pallid sturgeon, Scaphirhynchus albus: U.S. Geological Survey data release, https://doi.org/10.5066/P9OTQTH8.

Type: Related Primary Publication

Spawning, propagation, and recovery of the federally endangered pallid sturgeon (Scaphirhynchus albus) are complicated by morphological similarity between pallid sturgeon and their hybrids with sympatric shovelnose sturgeon. Current molecular markers cannot reliably distinguish among the species and backcrosses, and development of reliable disomic markers is complicated by an ancient whole genome duplication in the Scaphirhynchus ancestor. Thus, these data were generated to confirm 100% homozygous DNA and estimate DNA yield of sturgeon larvae artificially produced by using various milt treatments to disallow sperm genetic contribution, and various female egg donors. Specimens with 100% homozygous sturgeon DNA can be ultimately used in a future study to generate a reference DNA sequence that may be helpful in discerning the wild caught sturgeon that are pure species from those with multigenerational backcrosses.

• Columbia Environmental Research Center (CERC)
• USGS Data Release Products