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  3. qPCR Results for An Assessment of Naegleria fowleri in Grand Teton National Park, Wyoming

qPCR Results for An Assessment of Naegleria fowleri in Grand Teton National Park, Wyoming

Dates

Publication Date
Start Date
2016-03-10
End Date
2017-03-22

Citation

Kinsey, S.M., Wright, P.R., Barnhart, E.P., Mattioli, M., Murphy, J., Kahler, A., Cornman, R.S., Iwanowicz, D., Eddy, Z.N., Halonen, S., Peyton, B., Eldridge, S.L., and Brugger, C.L., 2022, qPCR Results for An Assessment of Naegleria fowleri in Grand Teton National Park, Wyoming: U.S. Geological Survey data release, https://doi.org/10.5066/P9UCQUD0.

Summary

In 2016, a multidisciplinary team from the U.S. Geological Survey Wyoming-Montana Water Science Center, National Park Service, The Centers for Disease Control and Prevention, and Montana State University’s Department of Microbiology and Immunology in cooperation with the Teton Conservation District, initiated a small study to document the presence or absence of Naegleria fowleri at several hot springs in Grand Teton National Park/John D. Rockefeller, Jr. Memorial Parkway. Over four sampling events in 2016-2017, Naegleria fowleri was detected in several of the Grand Teton National Park/John D. Rockefeller, Jr. Memorial Parkway hot springs for the first time. Additionally, the team found that the prevalence of Naegleria fowleri may be [...]

Contacts

Point of Contact :
Elliott P Barnhart
Process Contact :
Elliott P Barnhart
Originator :
Stacy M Kinsey, Peter R Wright, Elliott P Barnhart, Mia Mattioli, Jennifer Murphy, Amy Kahler, Robert S Cornman, Deborah Iwanowicz, Zachary N Eddy, Sandra Halonen, Brent Peyton, Sara L Eldridge, Cailin L Brugger
Metadata Contact :
Cailin L Brugger
Distributor :
U.S. Geological Survey - ScienceBase
SDC Data Owner :
Wyoming-Montana Water Science Center
USGS Mission Area :
Water Resources

Attached Files

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qPCR_Naegleria_fowleri_in_Grand_TetonNP_Metadata.xml
Original FGDC Metadata
View 		34.03 KB application/fgdc+xml
qPCR_Naegleria_fowleri_in_Grand_TetonNP_Controls.csv 2.95 KB text/csv
qPCR_Naegleria_fowleri_in_Grand_TetonNP.csv 82.07 KB text/csv

Purpose

Data were collected to document the presence or absence of Naegleria fowleri at several hot springs in Grand Teton National Park/John D. Rockefeller, Jr. Memorial Parkway using quantitative PCR (qPCR), culturing analysis, and high-throughput DNA sequencing. This will increase our knowledge of the environmental conditions and potential human-induced modifications (i.e. dam building within the hot springs) which are conducive to the growth of pathogenic microorganisms.

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DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9UCQUD0

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