The Nebraska Game and Parks Commission (NGPC) contracted the Rainwater Basin Joint Venture (RWBJV) to conduct a black-tail prairie dog colony inventory of Nebraska using 1-meter National Agriculture Imagery Program ( 2010) aerial imagery. We estimated the extent of each colony by placing polygon vertices on the furthest visible burrows. Burrows were then re-evaluated using sub-meter resolution imagery. Burrow delineations within 3 kilometers of one another were combined and considered a single colony resulting in 669 distinct colonies totaling 97,438 acres across the state of Nebraska.