Environmental DNA of selected terrestrial vertebrates and bacteria detected in the Platte River near Ashland, Nebraska 2019-22
Environmental DNA of selected terrestrial vertebrates and bacteria detected in the Platte River near Ashland, Nebraska 2019-22
Dates
Publication Date
2023-09-15
Start Date
2019-11-13
End Date
2022-09-13
Citation
Moser, M.T., 2023, Environmental DNA of selected terrestrial vertebrates and bacteria detected in the Platte River near Ashland, Nebraska 2019-22: U.S. Geological Survey data release, https://doi.org/10.5066/P94C4IEY.
Summary
Water samples from the Platte River were collected and analyzed for environmental DNA (eDNA). The U.S. Geological Survey (USGS) Nebraska Water Science Center (NEWSC) collected samples monthly from November 2019 to September 2022 at the Platte River near Ashland, Nebraska (USGS: 06801000). The USGS, in cooperation with the City of Lincoln, collected these samples. Water from the Platte River was collected as a series of weighted bottle subsamples that were taken from equal width increments across the river and composited into a sterilized 1-liter bottle. An aliquot of this composited sample was extracted using a sterile syringe and then filtered by the USGS NEWSC staff using filters provided by Jonah Ventures. These filters were frozen [...]
Summary
Water samples from the Platte River were collected and analyzed for environmental DNA (eDNA). The U.S. Geological Survey (USGS) Nebraska Water Science Center (NEWSC) collected samples monthly from November 2019 to September 2022 at the Platte River near Ashland, Nebraska (USGS: 06801000). The USGS, in cooperation with the City of Lincoln, collected these samples. Water from the Platte River was collected as a series of weighted bottle subsamples that were taken from equal width increments across the river and composited into a sterilized 1-liter bottle. An aliquot of this composited sample was extracted using a sterile syringe and then filtered by the USGS NEWSC staff using filters provided by Jonah Ventures. These filters were frozen and shipped to Jonah Ventures Laboratory in Boulder, Colorado. Any eDNA associated with 6 vertebrates (bovine, human, poultry, swine, canine, or sheep) or the Escherichia Coli bacteria were then extracted from the filter and processed using quantitative polymerase chain reaction (qPCR). These qPCR assays were chosen to identify and quantify the relative amount of eDNA for these different species that are present in the river near Ashland, Nebraska. Midway through the study effort (August 2021), a new preservative was included in the analytical method which may have introduced a step trend in some of the qPCR results. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
Click on title to download individual files attached to this item.
PlatteAshland_eDNA.xml Original FGDC Metadata
View
31.56 KB
application/fgdc+xml
PlatteAshland_eDNA.csv
4.06 KB
text/csv
Purpose
Data were collected to determine the presence and quantity of select eDNA genes in the Platte River near Ashland, Nebraska. Knowing the presence and quantity of selected eDNA in the water helps researchers to better understand possible contributors to fecal contamination in the current river conditions. A variety of flow conditions were targeted that included baseflow, runoff periods, and drought conditions.
