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Pd qPCR Interlaboratory Testing Results

Dates

Publication Date
Start Date
2019-01-01
End Date
2022-04-30

Citation

Alger, K.E., Mulrooney, D., Nemeth, N., Pabilonia, K., Parise, K., Sojonky, K., Adcock, K., Ballmann, A.E., Bell-Rogers, P., Cai, H., Cornelison, C., Foster, J. George, K.G., Joseph, T., and Lorch, J.M., 2023, Pd qPCR Interlaboratory Testing Results: U.S. Geological Survey data release, https://doi.org/10.5066/P93SXYL0.

Summary

These data were collected as part of a voluntary initiative to create a White-Nose Syndrome Diagnostic Laboratory Network among laboratories participating in research and surveillance for Pseudogymonascus destructans (Pd) - the fungal pathogen causing White-Nose Syndrome in bats. Pd_qPCR_InterlaboratoryLODdata.xlsx is raw qPCR data from multiple laboratories running serial dilutions of Pd gBlock in known concentrations for the collectively used Muller (2013) Pd qPCR assay. Pd_qPCR_InterlaboratoryResults_LOD.xlsx contains the data output for each laboratory from running a generic LOD/LOQ calculator script. the generic LOD/LOQ calculator script is available at:https://github.com/cmerkes/qPCR_LOD_Calc. Pd_qPCR_InterlaboratoryPTResults_PanelData.xlsx [...]

Contacts

Point of Contact :
Katrina E Alger
Process Contact :
Katrina E Alger
Originator :
Katrina E Alger, Donna Mulrooney, Nicole Nemeth, Kristy Pabilonia, Katy Parise, Ken Sojonky, Kayla Adcock, Anne E Ballmann, Patricia Bell-Rogers, Hugh Cai, Chris Cornelison, Jeffrey Foster, Kyle G George, Tomy Joseph, Jeffrey M Lorch
Metadata Contact :
Katrina E Alger, U.S. Geological Survey, WATER
Publisher :
U.S. Geological Survey
Distributor :
U.S. Geological Survey - ScienceBase
SDC Data Owner :
National Wildlife Health Center
USGS Mission Area :
Ecosystems

Attached Files

Click on title to download individual files attached to this item.

Pd_InterlaboratoryTesting_MetadataReconciled.xml
Original FGDC Metadata
View 		61.41 KB application/fgdc+xml
Pd_qPCR_InterlaboratoryResults_LODReconciled.csv 1.79 KB text/csv
Pd_qPCR_InterlaboratoryPTResults_StandardsReconciled.csv 4.84 KB text/csv
Pd_qPCR_InterlaboratoryPTResults_PanelResultsReconciled.csv 11.24 KB text/csv
Pd_qPCR_InterlaboratoryPTResults_PanelDataReconciled.csv 23.48 KB text/csv
Pd_qPCR_InterlaboratoryData_LODReconciled.csv 89.9 KB text/csv

Purpose

The data were collected as part of a voluntary interlaboratory diagnostic harmonization initiative to characterize performance of the Pd qPCR assay across laboratories and to establish network best practices.

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  • USGS Data Release Products

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Additional Information

Identifiers

Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P93SXYL0

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