Data to evaluate the Acute Toxicity of Lampricides to Non-target Species of Concern in the Lake Champlain Watershed. The toxicity of the lampricides TFM and TFM/1% Niclosamide to numerous non-target fishes of concern in the Great Lakes has been presented in previous studies. However, not many toxicity studies have been reported for Finger Lakes and Lake Champlain species of concern including: the fluted shell mussel (Lasmigona costata), the pocketbook mussel (Lampsilis ovata), the black sandshell mussel (Ligumia recta), the cylindrical papershell mussel (Anodontoides ferussacianus), young-of-year quillback (Carpiodes cyprinus), eastern sand darter (Ammocrypta pellucida), the channel darter (Percina copelandi), American brook lamprey (Lethenteron appendix), margined madtom (Noturus insignis), stonecat (Noturus flavus), and the mudpuppy (Necturus maculosus). We exposed these mussels, fishes and amphibians to lampricides for 12 hours at concentrations bracketing the minimum lethal concentrations (MLC) required to achieve successful control of larval sea lamprey (Petromyzon marinus), and plotted the resulting non-target mortalities (NOEC, LOEC, LC25, LC50) against the lampricide concentrations expressed as a multiple of the sea lamprey field determined LC99.9. Herein, we describe the results of toxicity tests that evaluate the potential acute impacts on the non-target organisms discussed above.