Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, limit of detection, 2019-2022
Dates
Start Date
2019-09-01
End Date
2022-10-30
Publication Date
2023-06-09
Citation
Peck, R.W., Munstermann, M., and Atkinson, C., 2023, Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, 2019-2022 (ver. 2.0, July 2023): U.S. Geological Survey data release, https://doi.org/10.5066/P9AEQWX0.
Summary
This data release documents sensitivity of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022.
Summary
This data release documents sensitivity of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022.
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qPCR Assay Limit of Detection.xml Original FGDC Metadata
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Related External Resources
Type: Related Primary Publication
Peck, R., Munstermann, M., Hayes, M., Atkinson, C., Beavers, S., Cupp, A., and Banko, P., 2023, Carbon dioxide (CO2) gas and eDNA assessment as tools for eradicating and monitoring invasive fish in anchialine pools in Hawai‘i: Management of Biological Invasions, v. 14, no. 4, p. 749–774, https://doi.org/10.3391/mbi.2023.14.4.11.
qPCR assays for detecting eDNA from invasive fish in anchialine pools were developed to provide tools for surveying anchialine pools for invasive fish and to document effectiveness of management actions to removed invasive fish from these pools. Results from this study are important because they will provide park managers with eDNA monitoring tools and a better understanding of how CO2 can be used to manage invasive fish in anchialine pools.
