Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, evaluation of assays, 2019-2022
Dates
Start Date
2019-09-01
End Date
2022-10-30
Publication Date
2023-06-09
Citation
Peck, R.W., Munstermann, M., and Atkinson, C., 2023, Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, 2019-2022 (ver. 2.0, July 2023): U.S. Geological Survey data release, https://doi.org/10.5066/P9AEQWX0.
Summary
This data release documents results of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies in pools with known fish populations and in pre and post-treatment samples from pools that were treated with carbon dioxide gas. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022.
Summary
This data release documents results of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies in pools with known fish populations and in pre and post-treatment samples from pools that were treated with carbon dioxide gas. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022.
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qPCR Field Evaluation of Assays.xml Original FGDC Metadata
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Field Evaluation of Assays.csv
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Related External Resources
Type: Related Primary Publication
Peck, R., Munstermann, M., Hayes, M., Atkinson, C., Beavers, S., Cupp, A., and Banko, P., 2023, Carbon dioxide (CO2) gas and eDNA assessment as tools for eradicating and monitoring invasive fish in anchialine pools in Hawai‘i: Management of Biological Invasions, v. 14, no. 4, p. 749–774, https://doi.org/10.3391/mbi.2023.14.4.11.
qPCR assays for detecting eDNA from invasive fish in anchialine pools were developed to provide tools for surveying anchialine pools for invasive fish and to document effectiveness of management actions to removed invasive fish from these pools. Results from this study are important because they will provide park managers with eDNA monitoring tools and a better understanding of how CO2 can be used to manage invasive fish in anchialine pools.
