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  4. Detection of Multiple Fish Species Using Environmental DNA (eDNA), Alaska 2018

Detection of Multiple Fish Species Using Environmental DNA (eDNA), Alaska 2018

Dates

Publication Date
Start Date
2016
End Date
2018

Citation

Menning, D.M., Pierson, B.J., Talbot, S.L., 2022, Detection of multiple fish species using environmental DNA (eDNA), Alaska 2018: U.S. Geological Survey data release, https://doi.org/10.5066/P9RU1RVN.

Summary

This release provides results of an environmental DNA (eDNA) study of fish identified in seven Alaskan lakes, three streams, and a positive control. Samples included replicates for multiple sampling dates and locations. The reference list and conserved primer sets to identify species present were developed using publicly available data (https://www.ncbi.nlm.nih.gov/genbank/). Highly conserved primers which could differentiate species of interest were developed for three portions of mtDNA genes (12S, 16S and COI).

Contacts

Point of Contact :
Alaska Science Center
Originator :
Damian M Menning, Barbara J Pierson, Sandra L Talbot
Metadata Contact :
Alaska Science Center
Distributor :
USGS ScienceBase Team
Publisher :
Alaska Science Center
SDC Data Owner :
Alaska Science Center
USGS Mission Area :
Ecosystems

Attached Files

Click on title to download individual files attached to this item.

fish_eDNA_Alaska_menning_metadata.xml
Original FGDC Metadata
View 		79.96 KB application/fgdc+xml
fish_eDNA_alaska_menning.csv 5.52 KB text/csv
fish_eDNAReferenceList_alaska_menning.csv 7.67 KB text/csv
fish_eDNA_Alaska_menning_metadata.html 156.03 KB text/html

Purpose

These data were collected to test the feasibility of using environmental DNA (eDNA) samples to identify the presence of fish species of interest in Alaskan lakes and streams.

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  • Alaska Science Center (ASC)

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DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9RU1RVN

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