This database contains physical and biological data for the Lower Colorado River Basin. The metrics include information on geology, hydrology, landcover, stewardship, topography, soil types and characteristics, stream characteristics, connectivity, and watershed position (information on where a stream segment or catchment is located in relationship to other segments or catchments; e.g. distance from a catchment to the mouth of the Colorado River, Shreve Link). In addition, there is a table with predicted probability of species occurrence for the connected stream segments and tables with the data used in those models. The species distribution models were completed for 18 native fish species and 21 non-native fish species. These data can be linked to a modified version of the National Hydrography Datasets for streams and watersheds and has been summarized to ecologically/biologically relevant spatial scales.