Identifying species occurrence in ecosystems of high conservation concern is especially important in the context of modern landscapes. This study investigated how stand-scale and landscape-scale factors affect priority birds associated with longleaf pine (Pinus palutris) ecosystems. Herein, I compared priority bird occupancy among 12 stand types throughout the historic range of longleaf pine. I found open pine stands positively influenced red-cockaded woodpecker (Picoides borealis) and Bachman’s sparrow (Peucaea aestivalis) occupancy, but were not significantly linked to northern bobwhite (Colinus virginianus) and brown-headed nuthatch (Sitta pusilla) occurrence. Landscape- and stand-scale factors affected red-cockaded woodpecker, Bachman’s sparrow, and brown-headed nuthatch occupancy. Northern bobwhite occupancy was influenced solely by landscape-scale factors. Red-cockaded woodpecker and Bachman’s sparrow were positively influenced by metrics associated with longleaf pine ecosystems suggesting they are effective indicator species. My analysis indicates that using this multi-scale approach is valuable to identifying areas on the landscape of conservation and restoration priority.