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  4. Histopathological assessment of the digestive gland of a freshwater snail (Lymnaea stagnalis) exposed to uranium

Histopathological assessment of the digestive gland of a freshwater snail (Lymnaea stagnalis) exposed to uranium

Dates

Publication Date
Start Date
2018-08-27
End Date
2018-09-05

Citation

Lankton, J.S., 2018, Histopathological assessment of the digestive gland of a freshwater snail (Lymnaea stagnalis) exposed to uranium: U.S. Geological Survey data release, https://doi.org/10.5066/P9ORSAKJ.

Summary

This dataset consists of measurements of the digestive gland in the freshwater snail Lymnaea stagnalis orally exposed to uranium. The objective of the study was to assess the morphological effects of uranium on the snail digestive gland by light microscopy. Laboratory-cultured L stagnalis were exposed to either synthetic or natural solid phases of uranium and a subset (6 controls and 6 exposed) were euthanized and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). The size of each sample, the length and width of digestive gland tubules (5 random glands per sample) and height of digestive cells (10 random cells per [...]

Contacts

Point of Contact :
Julia S Lankton
Originator :
Julia S Lankton
Metadata Contact :
Julia S Lankton
Publisher :
U.S. Geological Survey
Distributor :
U.S. Geological Survey - ScienceBase
SDC Data Owner :
National Wildlife Health Center
USGS Mission Area :
Environmental Health

Attached Files

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Lankton snails metadata.xml
Original FGDC Metadata
View 		22.37 KB application/fgdc+xml
epithelial height.csv 881 Bytes text/csv
Snail sample size.csv 221 Bytes text/csv
tubule size.csv 766 Bytes text/csv

Purpose

Previous studies performed in the Croteau laboratory suggest clinical effects (e.g. decreased feeding behavior) in snails orally exposed to uranium, but to our knowledge, pathological investigation of direct toxicosis has not been performed. This study is the first in a series of studies using histopathology as a technique for morphological assessment of toxicity.

Communities

  • National Wildlife Health Center
  • USGS Data Release Products

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9ORSAKJ

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