Skip to main content

In-situ incubation of light-and-dark bottles for the estimation of phytoplankton productivity in Levittown Lake, Toa Baja, Puerto Rico

Dates

Publication Date
Start Date
2010-04-22
End Date
2011-06-08

Citation

Soler-López, L.R. and Val-Merníz, N.A., 2022, Data for the Hydrologic and Water-Quality Characterization of Levittown Lake, Toa Baja, Puerto Rico, April 2010 – June 2011: U.S. Geological Survey data release, https://doi.org/10.5066/P9MC6JZ6.

Summary

This dataset contains tabulated-data of dissolved oxygen concentrations collected at Levittown Lake, Toa Baja, Puerto Rico. The standard light-dark method was used to determine the primary productivity in phytoplankton. The method consists in the in-situ incubation of two light-and-dark bottles for approximately four hours. Dissolved oxygen concentrations were estimated using the Winkler method. A total of twelve in-situ incubations were conducted on a monthly basis from April 2010 to June 2011. For each bottle incubated, two or three titrations were conducted and an average of the dissolved concentrations is calculated.

Contacts

Attached Files

Click on title to download individual files attached to this item.

Phytoplankton primary productivity.xlsx 18.67 KB application/vnd.openxmlformats-officedocument.spreadsheetml.sheet

Purpose

The purpose of the in-situ incubation of two light-and-dark bottles is to determine dissolved oxygen concentrations and ultimately, net changes in the dissolved oxygen concentrations by comparing dissolved oxygen concentrations in light and dark bottles with the initial bottle (fixed to avoid oxygen saturation). The main objectives of the investigation is to establish baseline data of the physical, chemical, biological, and bacteriological conditions of the Levittown Lake to define its seasonal variability over a 15-month period. The study included the lake’s main water body, the lake’s inlet/outlet channel (Rio El Cocal), and the Caño El Hato drainage canal. The principal primary producers in aquatic ecosystems include phytoplankton, periphyton, and macrophytes. Of these, phytoplankton often synthesizes the most organic matter in aquatic systems, although periphyton and macrophytes add substantial amounts of organic matter. The light (translucent) and dark (nontranslucent) bottle method, which can be used to determine net changes in the concentration of DO for a given volume of water within a given time interval (Dodds, and Whiles, 2010), was used to estimate gross primary productivity in the lake. This technique was used to analyze net primary productivity by phytoplankton in the water column. In addition, two diel (24-hour) studies were conducted to determine the aquatic net community primary productivity, which differentiates between phytoplankton, periphyton, and macrophyte productivity (Cornell and Klarer, 2008). The productivity associated only with periphyton and macrophytes can therefore be calculated by subtracting phytoplankton net primary productivity from community net primary productivity. References: Dodds, W., and Whiles, M., 2010, Aquatic chemistry and factors controlling nutrient cycling—Redox and O2, chap. 12 of Freshwater ecology, concepts & environmental applications of limnology (2d ed): Elsevier, p. 302–305. Cornell, L.P., and Klarer, D.M., 2008, Patterns of dissolved oxygen, productivity and respiration in Old Woman Creek estuary, Erie County, Ohio during low and high water conditions: The Ohio Journal of Science, v. 108, no. 3, p. 31–43.

Map

Communities

  • USGS Caribbean-Florida Water Science Center

Tags

Provenance

Data source
Input directly

Item Actions

View Item as ...

Save Item as ...

View Item...