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  5. Asian Carps (silver, bighead, black, & grass carps)
  6. Data release for validation of a portable eDNA detection kit for invasive carps

Data release for validation of a portable eDNA detection kit for invasive carps

Dates

Publication Date
Start Date
2017-08-29
End Date
2020-01-15

Citation

Kageyama, S.A., Hoogland, M.R., Tajjioui, T., Schreier, T.M., Erickson, R.A., and Merkes, C.M., 2022, Data release for validation of a portable eDNA detection kit for invasive carps: U. S. Geological Survey data release, https://doi.org/10.5066/P9FOS091.

Summary

This is the qPCR and loop-mediated isothermal amplification (LAMP) data in support of the article "Validation of a portable eDNA detection kit for invasive carps". There are four types of data contained in five files including: limit of detection analysis for each method (qPCR and LAMP), qPCR analysis and LAMP analysis of eDNA samples collected over time from ponds containing 3 or 33 Grass Carp (Ctenopharyngodon idella) mixed with thousands of baitfish. The standards and controls data collected with the eDNA sample analysis are split into separate files for each method to simplify the statistical analysis of the eDNA sample data.

Contacts

Point of Contact :
Stacie A Kageyama
Originator :
Stacie A Kageyama, Matthew R Hoogland, Tariq Tajjioui, Theresa M Schreier, Richard A Erickson, Christopher M Merkes
Metadata Contact :
Stacie A Kageyama
Publisher :
U.S. Geological Survey
SDC Data Owner :
Upper Midwest Environmental Sciences Center
USGS Mission Area :
Ecosystems
Distributor :
U.S. Geological Survey - ScienceBase

Attached Files

Click on title to download individual files attached to this item.

Metadata Wizard file_carp pond.xml
Original FGDC Metadata
View 		39.34 KB application/fgdc+xml
input_data_correct2.csv 9.54 KB text/csv
LAMP_LOD.csv 29.73 KB text/csv
LAMP-CTRLS.csv 15.84 KB text/csv
ND2_qPCR_LOD.csv 7.82 KB text/csv
qPCR STD-CONTROLS_Data.csv 124.68 KB text/csv
RAW_Data.csv 86.82 KB text/csv

Purpose

We collected the data in order to test the loop-mediated isothermal amplification (LAMP) primers we designed for the 4 invasive carp species, Hypophthalmichthys nobilis, H. molitrix, Ctenopharyngodon idella, and Mylopharyngodon piceus. We tested limit of detection for each species using synthetic DNA, and against qPCR primers for Grass carp in a mesocosm study with artificial ponds containing 3 and 33 Grass carp (C. idella).

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Communities

  • USGS Data Release Products
  • Upper Midwest Environmental Sciences Center (UMESC)

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Additional Information

Identifiers

Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9FOS091

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