The Bluestone sculpin (Cottus sp.) is a priority at-risk freshwater fish endemic to the Bluestone River system in Virginia and West Virginia. The species occurs in small, cool streams with gravel and rubble dominated substrates. The taxonomic status of the Bluestone sculpin is unresolved, and its range often overlaps with congeners, with which it may hybridize. To address the conservation status of the Bluestone sculpin, taxonomic analyses are needed to fully describe the species and evaluate the level of hybridization. Additionally, comprehensive surveys and genetic analyses are needed to determine the species range and population connectivity. Locating the species using eDNA may be a viable option.