2021 Organic Matter Research Lab Vectorized Fluorescence Data

Dates

Publication Date: 2023-11-06
Start Date: 2021-01-06
End Date: 2021-12-16

Citation

Oros, D.M., Hansen, A.M., Le, A.K., Bonham, B.G., Sanford, C.E., and Fleck, J.A., 2023, 2021 Organic Matter Research Lab Vectorized Fluorescence Data: U.S. Geological Survey data release, https://doi.org/10.5066/P9MM5KIV.

Summary

The USGS CAWSC Organic Matter Research Laboratory (OMRL) provides laboratory services and support to regional and national projects in the analysis of organic matter using the latest methods in absorbance and fluorescence spectroscopy. Optical measurements such as absorbance and fluorescence are used to gain insight into dissolved organic matter (DOM) composition, and can also serve as proxies for more expensive and difficult to obtain measurements. These techniques are relatively rapid and inexpensive and allow for the comprehensive tracking of DOM dynamics in aquatic ecosystems ranging from rivers and lakes to estuaries to open marine systems. Absorbance spectra and fluorescence matrices were simultaneously collected on filtered [...]

Summary

The USGS CAWSC Organic Matter Research Laboratory (OMRL) provides laboratory services and support to regional and national projects in the analysis of organic matter using the latest methods in absorbance and fluorescence spectroscopy. Optical measurements such as absorbance and fluorescence are used to gain insight into dissolved organic matter (DOM) composition, and can also serve as proxies for more expensive and difficult to obtain measurements. These techniques are relatively rapid and inexpensive and allow for the comprehensive tracking of DOM dynamics in aquatic ecosystems ranging from rivers and lakes to estuaries to open marine systems.

Absorbance spectra and fluorescence matrices were simultaneously collected on filtered water samples at room temperature (21 °C) in an acid-cleaned 1 cm quartz cuvette using a spectrofluorometer equipped with a charge-coupled device (CCD) (Aqualog®, Horiba Instruments, New Jersey, U.S.A.). Excitation and absorbance scans were performed using a double-grating monochrometer, a 150 W Xenon arc lamp, a 5 nm bandpass, and a 1 s integration time at wavelengths of 240-800 nm. Emission spectra were collected with a CCD at approximately 2.3 nm (4 pixel) intervals at wavelengths of 245–800 nm. Excitation and absorbance wavelengths were scanned from low to high energy (i.e., VIS to UV) to reduce UV exposure of the sample, thus limiting the effects of photobleaching during analysis.

Laboratory fluorescence measurements collected on the Aqualog can provide validation or verification of field-based instrument fluorescence measurements where the sensor arrays are encompassed by the full Aqualog array (Booth and others, 2023). This approach may be superior to the other correction approaches where interferences are large, especially in cases where the field sensor measurements constitute less than 10% of the fully corrected value. For projects that already collect discrete samples during field sensor maintenance visits, the cost and effort is minimal for the data quality benefit.

Data presented here were analyzed by the U.S Geological Survey California Water Science Center Organic Matter Research Lab, Sacramento CA. This release contains vectorized fluorescence data measured on the Aqualog ® fluorometer January-December 2021.

Contacts

Point of Contact :: Angela M Hansen
Process Contact :: Angela M Hansen
Originator :: Diana M Oros, Angela M Hansen, Anne K Le, Bryan G Bonham, Corrine E Sanford, Jacob A Fleck
Metadata Contact :: Angela M Hansen
Publisher :: U.S. Geological Survey
Distributor :: U.S. Geological Survey - ScienceBase
SDC Data Owner :: California Water Science Center
USGS Mission Area :: Water Resources

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2021 Organic Matter Research Lab Vectorized Fluorescence Data.xml Original FGDC Metadata	View	19.14 KB	application/fgdc+xml
2021_OMRL_Fluorescence_Data_Dictionary.csv		1.74 MB	text/csv
2021_OMRL_SampleMetadata.csv		127.48 KB	text/csv
2021_OMRL_Vectorized_Fluorescence_Data.csv		454.19 MB	text/csv

Purpose

The files provided in this Child page contain vectorized fluorescence data measured on the Aqualog ® fluorometer January-December 2021.

2021 Organic Matter Research Lab Vectorized Fluorescence Data

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