Cyanotoxin Concentrations in Extracts from Solid Phase Adsorption Toxin Tracking (SPATT) and Diffusive Gradients in Thin-Films (DGT) Samplers in Owasco Lake, Seneca Lake, and Skaneateles Lake, Finger Lakes Region, New York, 2019

Dates

Publication Date: 2024-06-06
Start Date: 2019-06-27
End Date: 2019-11-07

Citation

Stouder, M.D.W., Boyer, G.L., Carpenter, K.D., D'Angelo, E.M., Gorney, R.M., and Rosen, J.J., 2024, Cyanotoxin concentrations in extracts from solid phase adsorption toxin tracking (SPATT) and diffusive gradients in thin-films (DGT) samplers in Owasco Lake, Seneca Lake, and Skaneateles Lake, Finger Lakes Region, New York, 2019: U.S. Geological Survey data release, https://doi.org/10.5066/P9OZR89E.

Summary

This data release contains dissolved cyanotoxin concentrations for microcystins (MC), cylindrospermopsins (CYL), anatoxins (ATX), and saxitoxins (STX) assessed using enzyme-linked immunosorbent assays (ELISA) and liquid chromatography-mass spectrometry or tandem mass spectrometry (LC-MS and LC-MS/MS, respectively) in extracts from SPATT (n=95) and DGT samplers (n=10) deployed on three Finger Lakes (Owasco, Seneca, and Skaneateles) between June and November 2019. Relative percent contributions of microcystin congeners are also provided for samples analyzed by LC-MS and LC-MS/MS. Solid phase adsorption toxin tracking (SPATT) and diffusive gradients in thin-films (DGT) are types of passive environmental samplers containing porous synthetic [...]

Summary

This data release contains dissolved cyanotoxin concentrations for microcystins (MC), cylindrospermopsins (CYL), anatoxins (ATX), and saxitoxins (STX) assessed using enzyme-linked immunosorbent assays (ELISA) and liquid chromatography-mass spectrometry or tandem mass spectrometry (LC-MS and LC-MS/MS, respectively) in extracts from SPATT (n=95) and DGT samplers (n=10) deployed on three Finger Lakes (Owasco, Seneca, and Skaneateles) between June and November 2019. Relative percent contributions of microcystin congeners are also provided for samples analyzed by LC-MS and LC-MS/MS.

Solid phase adsorption toxin tracking (SPATT) and diffusive gradients in thin-films (DGT) are types of passive environmental samplers containing porous synthetic resin beads that adsorb and concentrate cyanotoxins over time. SPATT and DGT samplers are relatively inexpensive to construct and have been widely demonstrated to adsorb a range of cyanotoxins in different environments. These samplers can be deployed in water bodies for time periods ranging from hours to weeks and, upon extraction and analysis, provide a semi-quantitative, relative dissolved cyanotoxin concentration that is calculated based on the deployment length.

Contacts

Point of Contact :: Michael D Stouder
Originator :: Michael D Stouder, Gregory L Boyer, Kurt D Carpenter, Elisa M D'Angelo, Rebecca M Gorney, Joshua J Rosen
Metadata Contact :: Michael D Stouder
Publisher :: U.S. Geological Survey
USGS Mission Area :: Water Resources
SDC Data Owner :: New York Water Science Center
Distributor :: U.S. Geological Survey - ScienceBase

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FLX_SPATT_data_release_metadata_FINAL.xml Original FGDC Metadata	View	120.46 KB	application/fgdc+xml
TABLE_1_FLX_SPATT_ELISA.csv		27.11 KB	text/csv
TABLE_2_FLX_SPATT_LCMS_LCMSMS.csv		67.75 KB	text/csv
TABLE_3_FLX_DGT_ELISA.csv		3.26 KB	text/csv

Related External Resources

Type: Method

Kudela, R.M., 2011, Characterization and deployment of Solid Phase Adsorption Toxin Tracking (SPATT) resin for monitoring of microcystins in fresh and saltwater: Harmful Algae, v. 11, p. 117–125.	https://doi.org/10.1016/j.hal.2011.08.006
Roué, M., Darius, H., and Chinain, M., 2018, Solid Phase Adsorption Toxin Tracking (SPATT) Technology for the Monitoring of Aquatic Toxins: A Review: Toxins, v. 10, no. 4, p. 167.	https://doi.org/10.3390/toxins10040167
Lane, J.Q., Roddam, C.M., Langlois, G.W., and Kudela, R.M., 2010, Application of Solid Phase Adsorption Toxin Tracking (SPATT) for field detection of the hydrophilic phycotoxins domoic acid and saxitoxin in coastal California: SPATT for field detection of domoic acid: Limnology and Oceanography: Methods, v. 8, no. 11, p. 645–660.	https://doi.org/10.4319/lom.2010.8.0645
Barnard, M.A.; Chaffin, J.D.; Plaas, H.E.; Boyer, G.L.;Wei, B.; Wilhelm, S.W.; Rossignol, K.L.; Braddy, J.S.; Bullerjahn, G.S.; Bridgeman, T.B.; and others. 2021. Roles of Nutrient Limitation on Western Lake Erie CyanoHAB Toxin Production. Toxins 2021, v. 13, p. 47.	https://doi.org/10.3390/toxins13010047
Birbeck, J., Westrick, J., O’Neill, G., Spies, B., and Szlag, D. 2019. Comparative Analysis of Microcystin Prevalence in Michigan Lakes by Online Concentration LC-MS/MS and ELISA. Toxins 11(1), 13.	https://doi.org/10.3390/toxins11010013
Boyer, G.L., 2020, LCMS-SOP Determination of Microcystins in Water Samples by High Performance Liquid Chromatography (HPLC) with Single Quadrupole Mass Spectrometry (MS). protocols.io.	https://www.protocols.io/view/lcms-sop-determination-of-microcystins-in-water-sa-x54v9m7wmg3e/v1
D’Angelo, E., 2019, Development and evaluation of a sensitive, Diffusive Gradients in Thin-Films (DGT) method for determining microcystin-LR concentrations in freshwater and seawater: Harmful Algae, v. 89, p. 101668.	https://doi.org/10.1016/j.hal.2019.101668
Martin RM, Moniruzzaman M, Stark GF, Gann ER, Derminio DS, Wei B, Hellweger FL, Pinto A, Boyer GL and Wilhelm SW (2020) Episodic Decrease in Temperature Increases mcy Gene Transcription and Cellular Microcystin in Continuous Cultures of Microcystis aeruginosa PCC 7806. Front. Microbiol. 11:601864.	https://doi.org/10.3389/fmicb.2020.601864
US EPA, 2015. Method 545: Determination of Cylindrospermopsin and Anatoxin-a in Drinking Water by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry ( LC / ESI-MS / MS ).	https://www.epa.gov/sites/default/files/2017-10/documents/epa_815-r-15-009_method_545.pdf
Gold Standard Diagnostics, 2021(a), Anatoxin-a ELISA, Microtiter Plate User Guide.	https://www.goldstandarddiagnostics.com/pub/media/productattachments/files/a/n/anatoxin-a-elisa-user-guide-520060.pdf
Gold Standard Diagnostics, 2021(b), Cylindrospermopsin ELISA (Microtiter Plate) User Guide.	https://www.goldstandarddiagnostics.com/pub/media/productattachments/files/c/y/cylindrospermopsin-elisa-user-guide-522011.pdf
Gold Standard Diagnostics, 2021(c), Microcystin-ADDA ELISA (Microtiter Plate) User Guide.	https://www.goldstandarddiagnostics.com/pub/media/productattachments/files/m/i/microcystins_nodularins-adda-elisa-user-guide-520011.pdf
Gold Standard Diagnostics, 2021(d), Saxitoxin (PSP) ELISA, Microtiter Plate User Guide.	https://www.goldstandarddiagnostics.com/pub/media/productattachments/files/s/a/saxitoxins-elisa-user-guide-52255b.pdf

Type: Citation

Johnston, B.D., Gifford, S.R., Savoy, P.R., Finkelstein, K.M., and Stouder, M.D., 2023, Field data for an evaluation of sensors for continuous monitoring of harmful algal blooms in the Finger Lakes Region, New York, 2018 - 2020: U.S. Geological Survey data release, https://doi.org/10.5066/P9046YOS.	https://doi.org/10.5066/P9046YOS
Perkins, S.R., Stouder, M.D.W., and Beaulieu, K., 2021, Phytoplankton data from Owasco, Seneca, and Skaneateles Lakes, Finger Lakes Region, New York, 2019-2020 (ver. 2.1, June 2023): U.S. Geological Survey data release, https://doi.org/10.5066/P9TP9T1D.	https://doi.org/10.5066/P9TP9T1D

Purpose

Solid phase adsorption toxin tracking (SPATT) and diffusive gradients in thin-films (DGT) samplers were deployed to assess their utility as a tool in monitoring for the presence and relative concentrations of dissolved cyanotoxins in three low-nutrient Finger Lakes, where cyanotoxin concentrations in whole water are commonly below the detectable limit by enzyme-linked immunosorbent assays (ELISA). Extracts from passive samplers were preserved and analyzed according to ELISA kit manufacturer recommendations. A subset of extracts was chosen for additional analysis by liquid chromatography-mass spectrometry, tandem mass spectrometry (LC-MS and LC-MS/MS, respectively), or both. These data allow for comparisons of dissolved cyanotoxin concentrations from passive sampler extracts across multiple analytical methods and exploring impacts of ELISA preservatives on extracts analyzed by LC-MS and LC-MS/MS.

Cyanotoxin Concentrations in Extracts from Solid Phase Adsorption Toxin Tracking (SPATT) and Diffusive Gradients in Thin-Films (DGT) Samplers in Owasco Lake, Seneca Lake, and Skaneateles Lake, Finger Lakes Region, New York, 2019

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