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This dataset documents locations of and water temperature recorded by Hobo Water Temp Pro series loggers (Onset Computer Corp., Bourne, MA) deployed by the USGS Comprehensive Sturgeon Research Project at up to 24 locations in the Lower Missouri River (from the Gavins Point Dam near Yankton, South Dakota to its confluence with the Mississippi River near St. Louis, Missouri) and 13 selected tributaries (James River, Big Sioux River, Little Sioux River, Platte River (in Nebraska), Nishnabotna River, Nodaway River, Platte River (in Missouri), Kansas River, Grand River, Chariton River, Lamine River, Osage River, and Gasconade River) since 2005. Data document the site name, spatial location, temporal extent of water temperature...
Data consist of genotypes for pallid sturgeon and paddlefish broodstock and artificially-produced larvae at 19 sturgeon microsatellite loci and four paddlefish loci. Microsatellite genotypes allowed confirmation of homozygous sturgeon DNA and absence of paddlefish DNA in haploid gynogens. Data also consist of DNA yield estimates for haploid gynogens for proof of concept that haploid gynogens can provide sufficient DNA quantity for restriction site-associated DNA sequencing studies.
Lack of reproduction has been hypothesized to be a factor in the decline of the pallid sturgeon (Scaphirhynchus albus) through much of its range. Spawning habitat and behavior have only recently been documented on the Lower Missouri River, which comprises over 1,300 river kilometers of undammed river. This data consists of habitat measurements for ten pallid sturgeon spawning events from 2008-2013 that occurred in discrete reaches dispersed over hundreds of kilometers on the Lower Missouri River.
This dataset documents the number of normally developed hatched free embryos, abnormally developed hatched free embryos, and viable unhatched embryos from two laboratory trials (shovelnose sturgeon during April and pallid sturgeon during May 2016) in substrate conditions designed to simulate potential fates within a dynamic riverine environment. Trials were performed with substrate treatments that consisted of clean glass (control), gravel, medium-coarse sand, and fine sand-silt. Medium-coarse sand and fine sand-silt substrates were each tested three ways: unburied, partially buried, and fully buried (1–2 millimeter depth). Pallid sturgeon and shovelnose sturgeon embryos were exposed to trial conditions from the...
This dataset documents the length and developmental stage of pallid sturgeon and shovelnose sturgeon free embryos from the time of hatch through melanin plug expulsion (the approximate onset of exogenous feeding) reared in the laboratory at a constant temperature. Free embryos from 4 pallid sturgeon crosses and 7 shovelnose sturgeon crosses were reared separately in the laboratory at a mean temperature of 17.8 degrees Celsius (range 17.3–18.4 ). Beginning on the day of hatch, a minimum of three to six free embryos from each cross was randomly sampled and preserved in 10% neutral buffered formalin at 2 to 12-hour intervals, depending on age. Free embryos were microscopically examined and assigned a developmental...
Data consist of flow cytometry files (.FCS) that were generated in the analysis of larval pallid sturgeon specimens. Nuclei from produced larval sturgeon somatic cells were analyzed using flow cytometry to estimate genome size and assign ploidy. Freshly collected rainbow trout (Oncorhynchus mykiss), largemouth bass (Micropterus salmoides), or pallid sturgeon blood were used as external controls, whereby the fish blood samples were analyzed in separate tubes from the cells of prepared sturgeon larvae. All samples at 1 x 10^6 cells/mL were incubated in a propidium iodide solution to stain DNA, then analyzed by flow cytometry. Analysis software generated histograms from the experimental samples and the control standard...
This dataset documents the onset of developmental stages of pallid sturgeon free embryos from hatch through melanin plug expulsion, when reared in the laboratory at five nominal temperatures (14, 17, 20, 23, and 26 degrees Celsius, ° C). Onset of developmental stages were recorded in days post-hatch (DPH) and cumulative thermal units (CTU). CTU are calculated as the sum of water temperature, in ° C, during the time of exposure, in our case, from the time of hatch to the time of sample preservation. Pallid sturgeon transition through a series of brief developmental stages during a free-embryo period from hatch to melanin plug expulsion, which is regarded as the approximate onset of exogenous feeding. Detailed characteristics...
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