Filters: partyWithName: Adam J Sepulveda (X)
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We collected diets on Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri) and brown trout (Salmo trutta) above (Yellowstone cutthroat trout allopatry) and below (Yellowstone cutthroat trout sympatry with brown trout) a natural barrier in Duck Creek, Montana. We used gastric lavage methods to collect diets, and samples were placed in storage containers and taken back to our lab and frozen until sorting. All samples were sorted by Rhithron, Inc. (Missoula, MT) with individuals identified down to the order.
We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
This dataset contains simple sequence repeat genotypes from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA.
Positive and negative dreissenid mussel DNA quantitiative PCR results from environmental DNA water samples collected in Montana, Wisconsin and Minnesota to assess if environmental DNA can extend the seasonal window for dreissenid mussel early detection.
Categories: Data;
Tags: Flathead,
Missouri-Marias,
Pepin,
USGS Science Data Catalog (SDC),
Upper Mississippi,
This dataset contains molecular diagnostic results of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue collected from the inter-mountain West, USA.
Real-time PCR results of a round robin evaluation of 5 assays that target dreissenid mussel DNA. Water samples collected from waters with and without dreissenid mussels were analyzed using these five assays in four USGS laboratories. Samples from waters without dreissenid mussels were spiked with known amounts of dreissend DNA.
This data release contains molecular diagnostic results and molecular detection of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue and environmental DNA samples collected from the inter-mountain West, USA.
Field estimates of the abundance of rainbow trout in Washington and British Columbia were collected in concert with environmental DNA samples (eDNA) to evaluate if eDNA copy numbers correlated with abundance of trout. In addition, stream habitat data including channel units (pools, riffles), substrate, large woody debris, among others, were collected at sites.
Climate change may facilitate the expansion of non-native invasive species (NIS) in aquatic and terrestrial systems. However, empirical evidence remains scarce and poorly synthesized at scales necessary for effective management. We conducted a literature synthesis to assess the state of research on the observed and predicted effects of climate change on a suite of 398 aquatic and terrestrial NIS now present in or a major threat to aquatic and terrestrial ecosystems of the Pacific Northwest (PNW), USA and British Columbia. Surprisingly, very few studies (n = 15) have investigated the observed effects of climate change on the distribution, abundance, spread, or impact of the focal NIS, with only five studies focusing...
Categories: Publication;
Types: Citation;
Tags: Fish,
Northwest CASC,
Other Wildlife,
Plants,
Science Tools For Managers,
Field estimates of the abundance of two trout species (bull trout and westslope cutthroat trout) in Montana and rainbow trout in Washington and British Columbia were collected in concert with environmental DNA samples (eDNA) to evaluate if eDNA copy numbers correlated with abundance of trout. In addition, stream habitat data including channel units (pools, riffles), substrate, large woody debris, among others, were collected at sites.
The dataset includes measurements of stream habitat, fish abundance of westslope cutthroat trout and bull trout, and species-specific measures of environmental DNA (eDNA) from within the water. The data covers multiple streams in western Montana.
U.S. Geological Survey decision analysts and technical experts worked with representatives from the U.S. Bureau of Reclamation, Utah Division of Wildlife Resources, and Central Utah Water Conservancy District to predict the consequences of alternative actions following environmental DNA detections of dreissenid mussel eDNA in Jordanelle Reservor, Utah. This dataset provides the consequence and model inputs for each of the alternative actions under different levels of uncertainty.
Categories: Data;
Tags: Aquatic Biology,
Ecology,
Genetics,
Jordanelle Reservoir,
USGS Science Data Catalog (SDC),
Invasive alien species (IAS) are a rising threat to biodiversity, national security, and regional economies, with impacts in the hundreds of billions of U.S. dollars annually. Proactive or predictive approaches guided by scientific knowledge are essential to keeping pace with growing impacts of invasions under climate change. Although the rapid development of diverse technologies and approaches has produced tools with the potential to greatly accelerate invasion research and management, innovation has far outpaced implementation and coordination. Technological and methodological syntheses are urgently needed to close the growing implementation gap and facilitate interdisciplinary collaboration and synergy among...
Categories: Publication;
Types: Citation
The headwaters of the Columbia River Basin in the Northern Rocky Mountains region is widely recognized as a stronghold for native fish, containing some of the last remaining connected cold-water habitats for species such as the threatened bull trout and native westslope cutthroat trout. However, as temperatures rise, non-native invasive fish species could be poised to prosper in the region as conditions start to favor warm-water species over those that require cooler waters to thrive. The spread of invasive fish species has the potential to devastate native fish populations, stream habitats, and the local cultures and economies that depend on healthy aquatic ecosystems – including the region’s multi-billion dollar...
Categories: Project;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: 2018,
CASC,
Climate change,
Completed,
Data Visualization & Tools,
This dataset contains quantitative results from, and covariate attributes of, environmental DNA water samples collected across multiple rivers in 2023. Samples were analyzed for eDNA of round goby in the Hudson River (NY), westslope cutthroat trout (Oncorhynchus clarkii lewisi) in Cherry Creek (MT), lake trout (Salvelinus namaycush) and Spectaclecase mussels (Cumberlandia monodonta) in Big Piney River (MO), and rainbow trout (O. mykiss) and Western Pearlshell (Margaritifera falcata) in Loggers Creek (ID).
This dataset contains DNA sequences of two genomic regions, cytochrome oxidase subunit one and internally transcribed spacer region one, obtained from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA.
Environmental DNA results for March 2022 smallmouth bass surveillance in the Gardner River, Montana
Categories: Data;
Tags: Aquatic Biology,
Gardner River,
Montana,
Smallmouth Bass,
USGS Science Data Catalog (SDC),
U.S. Geological Survey decision analysts and technical experts worked with managers and stakeholders to predict the consequences of alternative actions following environmental DNA detections of invasive Najas minor eDNA in Sebago Lake, Maine, USA. This dataset provides the consequence and model inputs for each of the alternative actions under different levels of uncertainty.
Categories: Data;
Tags: Aquatic Biology,
Ecology,
Genetics,
Sebago Lake,
USGS Science Data Catalog (SDC),
Quantitative PCR data from mesocosm experiments and field evaluations comparing coarse filter-large water volume environmental DNA samples vs. fine filter-small water volume environmental DNA samples for detection and quantification of rainbow trout (Oncorhynchus mykiss) and bull trout (Salvelinus confluentus) DNA. Mesocosm experiments took place at the US Fish & Wildlife Service's Creston Fish Hatchery in Montana, 2016. Field surveys took place in the Flathead River basin of Montana, 2016.
The dataset contains qualitative detection and quantitative results of Esox Lucius DNA in environmental (e)DNA water samples collected from Miller Creek, Alaska and analyzed with quantitative PCR.
Categories: Data;
Tags: Alaska,
Aquatic Biology,
USGS Science Data Catalog (SDC),
biota,
environmental DNA
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