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Subsurface microbial (biogenic) methane production is an important part of the global carbon cycle and has resulted in natural gas accumulations in many coal beds worldwide. Laboratory experiments indicate coal beds can act as natural geobioreactors and produce additional low carbon renewable natural gas with algal or yeast compounds, yet the effectiveness of these nutrients in situ are unknown. This study uses down-well monitoring methods in combination with deuterated water (99.99% D2O) and a 200-liter injection of 0.1% yeast extract to stimulate and isotopically label newly generated methane. A total dissolved gas pressure sensor was placed down-well into the Flowers-Goodale coal bed at the USGS Birney Test Site...
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Data were collected to monitor geochemistry before and after an injection designed to stimulate microbial methanogenesis in the shallow Flowers-Goodale coal bed, near Birney in southeastern Montana. Waters from wells completed in the Flowers-Goodale, Nance, Knobloch, and Terret coalbeds at the Birney Test Site were sampled. Geochemical characterization of the water included non-purgeable dissolved organic carbon, acetate, major anions and cations, extractable hydrocarbons, and trace metals.
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This dataset includes the field measurements and laboratory analyses of surface water, seston, fish tissue, and sediment samples collected from Bighorn Lake, within Bighorn Canyon National Recreation area (BICA), during high flow (July 2015) and low flow (August 2016) conditions. The study area includes 7-9 sampling sites that follow a transect spanning the entire length of the reservoir from the Bighorn and Shoshone River inflows to the afterbay and tailwater below Yellowtail dam. Multiple samples were collected at each sampling site and usually included the top 2 cm of sediment at the sediment/water interface, 2 meters above the sediment/water interface, and 2 meters below the reservoir surface. Microbial samples...
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This study presents a simplified method and empirical relationships for determining organic matter thermal maturity using a portable Raman system equipped with a 785 nm laser, for analysis of crushed, whole-rock samples. Several sets of rocks comprised of shale and coal samples with various mineralogical composition, thermal maturity, total organic carbon (TOC), and age were used to test the method and build correlations between Raman band separation (RBS) values and traditional thermal maturity indicators; organic matter reflectance (Ro) and programmed temperature pyrolysis (Tmax) values. Several sample preparation methods were tested on cuttings material and standard deviation values for RBS were minimized by...
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In 2016, a multidisciplinary team from the U.S. Geological Survey Wyoming-Montana Water Science Center, National Park Service, The Centers for Disease Control and Prevention, and Montana State University’s Department of Microbiology and Immunology in cooperation with the Teton Conservation District, initiated a small study to document the presence or absence of Naegleria fowleri at several hot springs in Grand Teton National Park/John D. Rockefeller, Jr. Memorial Parkway. Over four sampling events in 2016-2017, Naegleria fowleri was detected in several of the Grand Teton National Park/John D. Rockefeller, Jr. Memorial Parkway hot springs for the first time. Additionally, the team found that the prevalence of Naegleria...
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In order to determine the innate microbial community of shale gas reservoirs and how they are impacted by hydraulic fracturing, this study analyzed biomass collected from produced water and rock from hydraulically fractured wells in the Utica Shale. The samples include rock chips from a drill core from one Utica well, produced water from that same Utica well, and produced water from 12 different Utica wells that had been in production between 1-5 years, spanning the oil and gas windows of SE Ohio. The samples were filtered for biomass, extracted, amplified, and 16S rRNA gene sequencing was performed on the Illumina MiSeq platform.


    map background search result map search result map Data for Biogeochemical and Physical Processes Controlling Mercury and Selenium Bioaccumulation in Bighorn Lake, Bighorn Canyon National Recreation Area, Montana and Wyoming, 2015-2016 Microbiology of the Utica Shale Injection of Deuterium and Yeast Extract at USGS Birney Field Site, Powder River Basin, Montana, USA, 2016-2020 qPCR Results for An Assessment of Naegleria fowleri in Grand Teton National Park, Wyoming Chemistry Data from the Birney Test Site, Montana, 2018-2020 Portable Raman spectroscopic analysis of bulk crushed rock Injection of Deuterium and Yeast Extract at USGS Birney Field Site, Powder River Basin, Montana, USA, 2016-2020 Chemistry Data from the Birney Test Site, Montana, 2018-2020 Data for Biogeochemical and Physical Processes Controlling Mercury and Selenium Bioaccumulation in Bighorn Lake, Bighorn Canyon National Recreation Area, Montana and Wyoming, 2015-2016 Microbiology of the Utica Shale Portable Raman spectroscopic analysis of bulk crushed rock