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Three SAS code files were used to analyze data for this study and they included: 1) code to compare the weight of fish distributed to the tanks, 2) code to compare the survival of fish by treatment groups, and 3) code to analyze the weight and condition of fish at exposure termination.
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
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Data and code release to evaluate a continuous-flow streamside toxicity test that was conducted with common logperch (Percina caprodes) and larval sea lamprey (Petromyzon marinus) to evaluate the risk posed by 4-nitro-3-(trifluoromethyl)phenol (TFM) sea lamprey control stream treatments to logperch. Logperch are a host to the parasitic glochidia life stage of the federally endangered snuffbox mussel (Epioblasma triquetra). Streams with an extant population of snuffbox must be treated before May 1st to prevent inadvertent take through TFM-related mortality of glochidia-infested fish. The concentration of TFM required to induce 99.9% mortality of sea lamprey was 6.52 mg/L and the concentration of TFM required to induce...
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
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This dataset contains the supplementary data to the dataset entitled "Assessments of the binary mixtures of four toxicants on Zebra Mussel (Dreissena polymorpha) mortality." These data describe the exposure conditions to include water chemistry before, during, and after exposures as well as mussel condition throughout the study. Monitored water chemistry parameters include dissolved oxygen, temperature, and pH. Periodic measurements of alkalinity, total hardness, and specific conductance where taken before each exposure period. Mussel condition was also assessed at the initiation of each exposure. Mussel condition included shell length and tissue dry weight.
Five R Markdown files (.Rmd) were created for the study entitled: Assessments of the binary mixtures of four toxicants on Zebra Mussel (Dreissena polymorpha) mortality. R codes were used to determine toxicant mixture ratios, summarize data, and analyze dose-response relationships in single toxicant and toxicant mixture trials. Files are described in the readme file in the order in which they were used for the study. Each R Markdown file has a complimentary R Script file (.R) with the same file name. All R Markdown files were created in RStudio version 1.2.1335 with R version 3.6.1 and Tidyverse version 1.3.0. Additional packages are listed in braces, “{},” with the corresponding R Markdown description.
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
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This dataset captures the Zebra Mussel dose-response to four independent toxicants and each binary mixture of those toxicants. Toxicants included EarthTec QZ, Clam-Trol CT-2 (syn. Spectrus CT1300), niclosamide, and potassium chloride. Each dose of toxicant was verified with either ICP-OES or UHPLC, depending on analyte, and comparison to a standard curve. Mortality was tabulated for each observation.
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Zequanox® is an EPA-registered biopesticide for controlling populations of dreissenid mussels (zebra and quagga mussels). Zequanox has demonstrated selective toxicity toward dreissenid mussels; however, a recent laboratory study indicated the potential for Zequanox to impact a non-target salmonid species. We assessed the potential exposure risk by observing the avoidance behavior of two representative species of cold-, cool-, and warm-water fish (lake trout, brook trout, lake sturgeon, yellow perch, fathead minnow, and bluegill). Fish were subjected to the maximum allowable concentration of Zequanox per the product label (100 mg/L active ingredient). Naïve juvenile fish (n = 30 per species) were individually observed...
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
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Zebra mussels (Dreissena polymorpha) have continued their spread within inland lakes and rivers in North America despite diligent containment and decontamination efforts by natural resource agencies and other stakeholders. Identification of newly infested waterways with early detection surveillance programs allows for rapid response zebra mussel eradication treatments in some situations. Previous eradication treatments have been conducted during times of variable water temperatures and temperature has been shown to influence the efficacy of molluscicides. Natural resource managers would benefit from knowledge regarding the impacts of water temperature and exposure duration on toxicity of molluscicides to zebra mussels....
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We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
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Bayluscide, the 2-aminoethanol salt of niclosamide (2′, 5-dichloro-4′-nitrosalicylanilide), is a pesticide that is used in conjunction with TFM (4-Nitro-3-(trifluoromethyl)phenol) to treat streams tributary to the Great Lakes that are infested with invasive parasitic sea lamprey (Petromyzon marinus). The application TFM with 0.5 to 2.0 percent (%) Bayluscide can significantly reduce the amount of TFM required to achieve effective control. The current formulation of Bayluscide used in combination TFM during stream treatments conducted the Great Lakes Fishery Commission binational Sea Lamprey Control Program is an emulsifiable concentrate (EC). The Bayluscide EC formulation is highly effective; however, it degrades...
Alternatives to chemicals for controlling dreissenid mussels are desirable for environmental compatibility, but few alternatives exist. Previous studies have evaluated the use of electrified fields for stunning and/or killing planktonic life stages of dreissenid mussels, however, the available literature on the use of electrified fields to control adult dreissenid mussels is limited. We evaluated the effects of sinusoidal alternating current (AC) and square- wave pulse direct current (PDC) exposure on the survival of zebra mussels at water temperatures of 10, 15, and 22°C. Peak voltage gradients of ~ 17 and 30 Vp/cm in the AC and PDC exposures, respectively, were continuously applied for 24, 48, or 72 h. Peak power...
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
Two SAS code files were used to analyze data for this study and they included: 1) code to compare the condition of unique cohorts of zebra mussel test animals at 3 time points (pre-exposure, exposure termination, and post-exposure termination), and 2) code to predict the lethal concentration of test articles required to cause 50 and 99% mortality (LC50 and LC99, respectively) for each toxicant, exposure duration, and temperature combination.
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Controlling larval sea lamprey (Petromyzon marinus) in Great Lakes tributaries with 4-nitro-3-(trifluoromethyl)phenol (TFM, a pesticide) stream treatments reduces the number of spawning-phase sea lamprey, an invasive species capable of collapsing Great Lakes fisheries. An important component of stream treatments is ensuring that the proper amount of TFM is applied that will effectively control sea lamprey populations and minimize the effects on non-target species. In this study, replicated streamside bioassays were conducted in May, July, and September at six sea lamprey infested rivers in Michigan to determine potential seasonal changes in sensitivity of lamprey to TFM. Larvae (≥ 60 mm) were collected with backpack...
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Zebra mussels (Dreissena polymorpha Pallas 1771) are a tenacious aquatic invasive species in the United States and new infestations can rapidly expand into dense colonies. Zebra mussels were first reported in Marion Lake (Dakota County, Minnesota) in September 2017 and surveys indicated the infestation was likely isolated near the public boat access. A 2.4-hectare area, containing the known zebra mussel infestation, was enclosed and treated for nine days with EarthTec QZ®, a copper-based molluscicide, in an attempt to eradicate the zebra mussels. We conducted an on-site bioassay to confirm treatment efficacy. The bioassay was conducted in mobile assay trailer that received a continuous flow of treated lake water...


map background search result map search result map Bioassay Verification of a Zebra Mussel (Dreissena polymorpha) Eradication Treatment: Data Assessment of uncontained Zequanox applications in a Midwestern lake data Plots - Assessment of uncontained Zequanox applications in a Midwestern lake data Unionid Shape File Info for Assessment of uncontained Zequanox applications in a Midwestern lake Zequanox Concentration - Assessment of uncontained Zequanox applications in a Midwestern lake data Zebra Mussel Density - Assessment of uncontained Zequanox applications in a Midwestern lake data Zebra Mussel Mortality - Assessment of uncontained Zequanox applications in a Midwestern lake data Data and Code Release: Determination of Larval Sea Lamprey (Petromyzon marinus) Seasonal Sensitivity Differences to Lampricide Treatment Data and code release—Comparative toxicity of emulsifiable concentrate and suspension concentrate formulations of 2′,5-dichloro-4′-nitrosalicylanilide ethanolamine salt Data and Code Release:Technical Assistance Bioassay to Compare Sea Lamprey and Logperch Sensitivity to TFM Unionid Shape File Info for Assessment of uncontained Zequanox applications in a Midwestern lake Zequanox Concentration - Assessment of uncontained Zequanox applications in a Midwestern lake data Zebra Mussel Mortality - Assessment of uncontained Zequanox applications in a Midwestern lake data Zebra Mussel Density - Assessment of uncontained Zequanox applications in a Midwestern lake data Plots - Assessment of uncontained Zequanox applications in a Midwestern lake data Assessment of uncontained Zequanox applications in a Midwestern lake data Bioassay Verification of a Zebra Mussel (Dreissena polymorpha) Eradication Treatment: Data Data and Code Release: Determination of Larval Sea Lamprey (Petromyzon marinus) Seasonal Sensitivity Differences to Lampricide Treatment Data and Code Release:Technical Assistance Bioassay to Compare Sea Lamprey and Logperch Sensitivity to TFM Data and code release—Comparative toxicity of emulsifiable concentrate and suspension concentrate formulations of 2′,5-dichloro-4′-nitrosalicylanilide ethanolamine salt