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The dataset contains Esherichia coli and quantitative polymerase chain reaction data for microbial source tracking markers in the Chattahoochee River and streams within the Chattahoochee River National Recreation area from samples collected in the winter and summer of the federal fiscal years 2016 and 2017. Six water samples were collected from three sites on the Chattahoochee River each season. Eleven stream sites and one additional site on the Chattahoochee River were sampled once each season, with the exception of two sites, which were not sampled in winter FY16.
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The table contains oxide and cation compositions of olivine grains from drill core of the Duluth Complex, Minnesota. The oxides were determined by electron probe microanalysis (EPMA) and are reported in weight percent (wt%). The cations were calculated based on the oxide compositions and are reported in mole percent (mol%). Olivine is a magnesium iron orthosilicate mineral with the formula (Mg2+, Fe2+)2SiO4. Olivine forms a solid solution series with magnesium (Mg) at one end and iron (Fe) at the other. Forsterite (Mg2SiO4) is the magnesium endmember and fayalite (Fe2SiO4) is the iron endmember. Minor elements can substitute into the crystal structure, such as manganese (Mn) and nickel (Ni). To better understand...
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Little is known about the underlying mechanisms governing uranium (U) bioaccumulation in aquatic insects, especially the influence of surface adsorption on the measured U concentrations (Henry et al. 2020). U.S. Geological Survey scientists are conducting experiments to parameterize conditional rate constants for aqueous U uptake and to quantify desorption of weakly bound U from insect's integument. Henry, Brianna L., Marie-Noële Croteau, David M. Walters, Janet L. Miller, Daniel J. Cain, and Christopher C. Fuller. Uranium bioaccumulation dynamics in the mayfly Neocloeon triangulifer and application to site-specific prediction. Environmental Science & Technology 54, no. 18 (2020): 11313-11321.
Water, bed sediment, and biota were sampled in selected streams from Butte to near Missoula, Montana, as part of a monitoring program in the upper Clark Fork Basin of western Montana. The sampling program was led by the U.S. Geological Survey (USGS), in cooperation with the U.S. Environmental Protection Agency.
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In a study conducted by the U.S. Geological Survey (USGS) and the New Hampshire Department of Environmental Services, detectable concentrations of per- and polyfluoroalkyl substances (PFAS) were found in the soil at every site despite targeting locations with no known PFAS sources (Santangelo and others, 2022). The widespread distribution of PFAS concentrations in New Hampshire has since sparked critical interest into understanding whether recharge to groundwater contains significant concentrations of PFAS after infiltration through soils. To address this concern, the USGS implemented a pilot study designed to evaluate whether PFAS infiltrate through shallow soil into shallow groundwater. Five sites were selected...
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A method was described for determination of trace levels of silicon by inductively coupled plasma mass spectrometry (ICP-MS) from hydrofluoric (HF) digests of biological samples. The volatility of silicon in HF digests was examined with synthetic solutions as well as soil and mine waste samples. A digestion scheme was optimized to achieve complete dissolution, avoid loss of volatile silicon species and produce sample solutions that can be directly analyzed by ICP-MS. The performance of the method performance was validated by analysis of a number plant and tissue certified reference materials for silicon by ICP-MS. The method was then applied to the determination of silicon from lung biopsy tissues obtained from...
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Pollen was measured in ambient air by several methods and in wet atmospheric deposition samples at three monitoring sites in the National Atmospheric Deposition Program (NADP) National Trends Network. A method for counting pollen on filters was developed and provided pollen counts for NADP atmospheric wet-deposition samples and high-volume ambient air samplers (HVAS) for comparison with co-located traditional microscopy and PollenSense sensor counting methods during the 2021 pollen season. Air and precipitation samples were collected by the NADP and analyzed by the Wisconsin State Laboratory of Hygiene, in Madison, Wisconsin and Aerobiology Research Laboratories (Canada). Daily data were obtained from online summaries...
Flea abundance and body condition data for black-tailed prairie dogs on sites treated and not treated with “FipBit” fipronil pellets. We sampled prairie dogs at Conata Basin, Buffalo Gap National Grassland, and Badlands National Park, South Dakota, USA. We sampled fleas from live-trapped prairie dogs during June–October 2018, May–October 2019, and July-August 2020. Prairie dogs were sampled before and after FipBit treatments. We anesthetized each prairie dog with isoflurane and combed it thoroughly for 30 seconds to dislodge fleas, which fell into a plastic bin. Fleas were counted and allowed to recover from anesthesia and placed back on prairie dogs, to minimize any removal effect. We weighed prairie dogs with...
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Data from study assessing the utility of knowledgebase-leveraging of comprehensive environmental-contaminant-exposure datasets by comparing biological effects predicted on the basis of target chemical analyses with measured biological effects in corresponding split water samples.
This work is part of a study of the immunological effects of exposure to alternative flame retardants in avian species. For the pathology portion of the study, hatchling American kestrels (Falco sparverius) were exposed to the flame retardant isopropyl triphenyl phosphate (ITP) and then challenged with a synthetic analogue of viral double-stranded RNA, polyinosinic:polycytidylic acid (poly I:C). Control birds were challenged with vehicle only or vehicle and poly I:C. At euthanasia, spleen, thymus, and bursa of Fabricius were collected and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). Quantitative...
Human-use pharmaceutical, pesticide, and wastewater indicator compounds were analyzed at the U.S. Geological Survey, National Water Quality Laboratory, Denver, Colorado, in wadeable streams in 4 Regional Stream Quality Assessments: Northeast (NESQA), Southeast (SESQA), Pacific Northwest (PNSQA) and California (CSQA). Multiple (with few exceptions) samplings occurred at each site, during base flow, between 2014 and 2017. Sites were located in the headwaters of perennial, wadeable streams in urban and agricultural watersheds. Site selection and methodology for each assessment can be found in Van Meter and others (2015), Sheibley and others (2015), Van Meter and others (2017), Coles and others (2016), Van Meter and...
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Adult walleye (10 females and 10 males) were caught in Fox River (Wisconsin) during April 2014. Total length, weight, and age were determined for each of the walleye. Whole-fish homogenates were prepared during 2017. Lipid concentration and polychlorinated biphenyl (PCB) congener concentrations were determined in each of the homogenates during 2017-2018. For each sample, PCB congener concentrations were summed to yield total PCB concentration. All lipid and PCB congener terminations were made at the University of Michigan School of Public Health in Ann Arbor, Michigan.
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The data set includes the macroinvertebrate metrics calculated by IDAS software (Cuffney 2003, USGS OFR 03-172) for the Midwest Stream Quality Assessment (MSQA). Invert data is taxonomic data that has been summarized into variables called metrics that summarize various components of the taxonomic data. The original taxonomic data comes from USGS BioData.
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Lead and zinc were mined in the Tri-State Mining District (TSMD) of southwest Missouri, northeast Oklahoma, and southeast Kansas for more than 100 years. The effects of mining on the landscape are still evident, nearly 50 years after the last mine ceased operation. The legacies of mining are the mine waste and discharge of groundwater from underground mines. The mine-waste piles and underground mines are continuous sources of trace metals (primarily lead, zinc, and cadmium) to the streams that drain the TSMD. Many previous studies characterized the horizontal extent of mine-waste contamination in streams but little information exists on the depth of mine-waste contamination in these streams. Characterizing the vertical...
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This dataset contains tabular data for U.S. Environmental Protection Agency’s (EPA) Clean Watersheds Needs Survey (CWNS) wastewater treatment facility information. Literature-based average total nitrogen and total phosphorous concentrations were substituted for wastewater treatment levels reported for each facility because little or no concentration data is reported in the CWNS. Concentrations were based on the treatment levels of raw, primary, secondary, and advanced. Concentration and effluent flow-value data gaps were ‘filled in’ through simple linear assumptions, based on each facility’s responses to the survey in years bracketing the data gap or were based on an un-bracketed single year survey response. Unlike...
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On July 26, 2017, a solution containing approximately 200 kilograms of dissolved sodium bromide was added to the the flooded underground shaft of the Minnesota Mine, near Empire, Colorado. This addition was conducted as part of an effort to determine the degree to which the mine shaft is hydrologically connected to a series of downgradient seeps and springs. These seeps and springs discharge from the hillside and enter Lion Creek, an acidic, metal-rich stream that lies immediately to the west of the Minnesota Mine. A water quality sonde and pressure transducer deployed within the shaft recorded temperature, specific conductance, and stage before, during, and after the addition. The downgradient seeps and springs...
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The rusty-patched bumble bee (Bombus affinis) is a listed species under the Endangered Species Act of the United States. While understood to be a highly generalist forager, little is known on the role that limited floral resources or shifting floral community composition could have played in B. affinis decline. Determining which floral species provide suitable B. affinis forage could assist conservation efforts where B. affinis persists, identify floral species for restoration efforts, or highlight regions to search for cryptic populations.
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A seminal question in ecotoxicology is the extent to which contaminant exposure evokes prolonged effects on physiological function and fitness. A series of studies were undertaken with American kestrels ingesting environmentally realistic concentrations of the second-generation anticoagulant rodenticide (SGAR) brodifacoum (BROD). Kestrels fed BROD at 0.3, 1.0 or 3.0 µg/g diet wet wt for 7 d exhibited dose-dependent hemorrhage, histopathological lesions and coagulopathy (prolonged prothrombin and Russell’s viper venom times). Following termination of a 7 d exposure to 0.5 µg BROD/g diet, prolonged blood clotting time returned to baseline values within a week, but BROD residues in liver and kidney (terminal half-life...
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A heterologous thrombin clotting time assay (TCT) was used to measure the time for conversion of fibrinogen to fibrin using commercially available reagents. Human reference material included in the kit was diluted with imidazole buffered saline (IBS; 0.0125M imidazole-0.109 M sodium chloride, pH7.4) to generate a standard curve. Each kestrel plasma sample was thawed at 37ºC and diluted with IBS, and following incubation at 37ºC, the reaction was then initiated by the addition of bovine thrombin reagent supplied with the assay kit, with clotting time measured to 0.1 s. Fibrinogen concentration was determined in a single assay for each of the 3 study trials, with reference samples interspersed among study samples....


map background search result map search result map Metals and Other Constituent Concentrations in Sediment of Selected Streams Draining the Tri-State Mining District, Missouri, Oklahoma, and Kansas, 2011–12 National USEPA Clean Watershed Needs Survey WWTP nutrient load data 1978 to 2012 Multi-stressor Predictive Models of Invertebrate Condition in the Corn Belt, U.S.A. Oxide and Cation Compositions of Olivine Grains from Drill Core in the Duluth Complex, Minnesota Minnesota Mine Shaft Tracer Data, Lion Creek Watershed near Empire, Colorado, July-November, 2017 Chemical-Gene and Chemical-Pathway Interactions Predicted for Chemicals Detected in the USGS-USEPA National Streams Pilot Study Based on Effects Data in the Comparative Toxicogenomics Database (CTD) Polychlorinated biphenyl concentrations in adult walleye from the Fox River (Wisconsin) population, 2014 Microbial Source Tracking Marker Concentrations in the Chattahoochee River National Recreation Area Watershed in 2015-2017, Georgia, USA Brodifacoum toxicity in American kestrels (Falco sparverius) with evidence of increased hazard upon subsequent anticoagulant rodenticide exposure 4. Results of Blood Clotting Assays Water-Quality, Bed-Sediment, and Invertebrate Tissue Trace Element Concentrations for Tributaries in the Clark Fork Basin, Montana, October 2017 - September 2018 Rusty Patched Bumble Bee Pollen Metagenome Flea abundance and body condition data for black-tailed prairie dogs on sites treated and not treated with “FipBit” fipronil pellets, South Dakota, 2018-2020 Concentrations of Pesticide, Pharmaceutical, and Organic Wastewater Contaminants from a Multi-Regional Assessment of Wadeable USA Streams, 2014-17 Data acquired in laboratory experiments conducted with the stonefly Zapada sp. and the ephemerellid mayflies Drunella sp. and Ephemerella tibialis to characterize uptake and surface adsorption after short aqueous exposures to uranium, 2017-2019 Lead (Pb) in Bald and Golden Eagles from 38 United States, USA, 2010-2018 National Atmospheric Deposition Program Pollen Study Data for 2021 Pollen Season Pilot Study of Per- and Polyfluoroalkyl Substances (PFAS) Infiltration to Shallow Groundwater Through Selected Soils in New Hampshire, 2023 Minnesota Mine Shaft Tracer Data, Lion Creek Watershed near Empire, Colorado, July-November, 2017 Brodifacoum toxicity in American kestrels (Falco sparverius) with evidence of increased hazard upon subsequent anticoagulant rodenticide exposure 4. Results of Blood Clotting Assays Flea abundance and body condition data for black-tailed prairie dogs on sites treated and not treated with “FipBit” fipronil pellets, South Dakota, 2018-2020 Metals and Other Constituent Concentrations in Sediment of Selected Streams Draining the Tri-State Mining District, Missouri, Oklahoma, and Kansas, 2011–12 Polychlorinated biphenyl concentrations in adult walleye from the Fox River (Wisconsin) population, 2014 Water-Quality, Bed-Sediment, and Invertebrate Tissue Trace Element Concentrations for Tributaries in the Clark Fork Basin, Montana, October 2017 - September 2018 Pilot Study of Per- and Polyfluoroalkyl Substances (PFAS) Infiltration to Shallow Groundwater Through Selected Soils in New Hampshire, 2023 Oxide and Cation Compositions of Olivine Grains from Drill Core in the Duluth Complex, Minnesota Multi-stressor Predictive Models of Invertebrate Condition in the Corn Belt, U.S.A. National Atmospheric Deposition Program Pollen Study Data for 2021 Pollen Season Rusty Patched Bumble Bee Pollen Metagenome Data acquired in laboratory experiments conducted with the stonefly Zapada sp. and the ephemerellid mayflies Drunella sp. and Ephemerella tibialis to characterize uptake and surface adsorption after short aqueous exposures to uranium, 2017-2019 Concentrations of Pesticide, Pharmaceutical, and Organic Wastewater Contaminants from a Multi-Regional Assessment of Wadeable USA Streams, 2014-17 National USEPA Clean Watershed Needs Survey WWTP nutrient load data 1978 to 2012 Chemical-Gene and Chemical-Pathway Interactions Predicted for Chemicals Detected in the USGS-USEPA National Streams Pilot Study Based on Effects Data in the Comparative Toxicogenomics Database (CTD) Lead (Pb) in Bald and Golden Eagles from 38 United States, USA, 2010-2018