Denise M Akob

Discovery of Two Biological Mechanisms for Acetylene Metabolism in a Single Organism

Acetylene fermentation assays, nitrogen fixation assays, and growth studies were performed with Pelobacter sp. strain SFB93 and Pelobacter acetylenicus DSM3246. Data includes concentrations of acetylene and ethylene over time, and growth measured with OD680 and cell counts.

Categories: Data; Types: Citation; Tags: Ecology, Environmental Health, Genetics, Geochemistry, NA, All tags... USGS Science Data Catalog (SDC), Water Quality, Water Resources, acetylene, bacteria, nitrogen, Fewer tags

Microbial Populations in PFHxSAm (perfluorohexane sulfonamido propyl amine) Biodegradation Microcosms

Water resources around the world are contaminated with per- and polyfluoroalkyl substances (PFAS) due to releases from point sources on military installations, fire training centers, and chemical manufacturing sites. Non-point sources have also been identified including wastewater effluent, landfills, and biosolids application. PFAS are a major concern to myriad stakeholders as some are known to bioaccumulate, they have eco-toxicity effects, and they are highly recalcitrant. PFAS are often called “forever chemicals” due to their environmental persistence but many precursor PFAS are transformed in the environment by microbes. Recent work has shown that PFAS can be biologically degraded in laboratory studies, but...

Categories: Data; Tags: Barnstable County, Cape Cod, DNA sequencing, Environmental Health, Hydrology, All tags... Massachusetts, PFAS, USGS Science Data Catalog (SDC), Water Quality, biodegradation, biota, environment, laboratory experiments, microbes, microbiology, time series analysis, time series datasets, Fewer tags

Per- and polyfluoroalkyl substances (PFAS) and volatile organic compounds measured in laboratory microcosm experiments with soil from Fort Drum, New York

Anaerobic microcosm experiments were conducted in April-May 2018 with PFAS-contaminated soil from a U.S. Army installation (Fort Drum, New York) and simulated groundwater. All microcosms, except for a live sediment control, were amended with perfluorooctanesulfonate (PFOS), perfluorooctanoate (PFOA), and 6:2 fluorotelomer sulfonate (6:2 FtS). Replicate treatments were prepared with and without bioaugmentation with the WBC-2 dehalogenating culture and with and without addition of chlorinated volatile organic compounds (1,1,2,2-tetrachloroethane and trichloroethylene). Two additional treatments were prepared containing granular activated carbon. All microcosms were prepared in duplicate and sacrificed for sampling....

Categories: Data; Tags: Environmental Health, Fort Drum, Genetics, Jefferson County, New York, All tags... New York, PFAS, USGS Science Data Catalog (SDC), Water Quality, bacteria, bacteria, bacterial digestion of pollutants, biodegradation, biogeochemical cycling, biological laboratory methods, environment, laboratory experiments, reductive dehalogenation, sediment-pollutant interaction, Fewer tags

Growth Characteristics of Syntrophotalea acetylenivorans, strain SFB93, sp. nov., a diazotrophic, acetylenotrophic anaerobe isolated from San Francisco Bay intertidal sediments

Data supporting the establishment of Syntrophotalea acetylenivorans SFB93 as a new microbial species. Data release includes growth curve and preferred growth conditions for the species.

Categories: Data; Tags: Aquatic Biology, Environmental Health, USGS Science Data Catalog (SDC), Water Quality, Water Resources, All tags... bacteria, bacteriology, biota, biota, culturing (specimens), microbiology, Fewer tags

Data on the Enrichment and Isolation of the Acetylenotrophic and Diazotrophic Isolate Bradyrhizobium sp. strain I71 (ver 2.0, September 2022)

Acetylene (C2H2) is a molecule rarely found in nature, with few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs, however we hypothesized that there may be yet unrecognized diversity of acetylenotrophs in nature. In this study, we expanded this diversity by isolating an aerobic acetylenotroph, Bradyrhizobium sp. strain I71, from trichloroethene (TCE)-contaminated soils undergoing bioremediation. TCE-contaminated soils from the NASA Ames Research Center in California were used to establish soil microcosms with acetylene as the primary carbon substrate and acetylene...

Categories: Data, Data Release - Revised; Tags: California, Moffett Field Naval Air Station (historical), USGS Science Data Catalog (SDC), bacteria, bacteriology, All tags... biota, culturing (specimens), environmental DNA, gas chromatography, laboratory experiments, microbiology, Fewer tags