Data on the Enrichment and Isolation of the Acetylenotrophic and Diazotrophic Isolate Bradyrhizobium sp. strain I71 (ver 2.0, September 2022)
Dates
Publication Date
2021-04-13
Time Period
2018-10-11
Last Revision
2022-09-12
Citation
Baesman, S.M., Klein, E., Andrews, R., Shrestha, Y., Bushman, T.J., Sutton, J.M., and Akob, D.M., 2021, Data on the Enrichment and Isolation of the Acetylenotrophic and Diazotrophic Isolate Bradyrhizobium sp. strain I71 (ver 2.0, September 2022): U.S. Geological Survey data release, https://doi.org/10.5066/P9DUG9O3.
Summary
Acetylene (C2H2) is a molecule rarely found in nature, with few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs, however we hypothesized that there may be yet unrecognized diversity of acetylenotrophs in nature. In this study, we expanded this diversity by isolating an aerobic acetylenotroph, Bradyrhizobium sp. strain I71, from trichloroethene (TCE)-contaminated soils undergoing bioremediation. TCE-contaminated soils from the NASA Ames Research Center in California were used to establish soil microcosms with acetylene as the primary carbon substrate and acetylene uptake was tracked [...]
Summary
Acetylene (C2H2) is a molecule rarely found in nature, with few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs, however we hypothesized that there may be yet unrecognized diversity of acetylenotrophs in nature. In this study, we expanded this diversity by isolating an aerobic acetylenotroph, Bradyrhizobium sp. strain I71, from trichloroethene (TCE)-contaminated soils undergoing bioremediation. TCE-contaminated soils from the NASA Ames Research Center in California were used to establish soil microcosms with acetylene as the primary carbon substrate and acetylene uptake was tracked over time and reported in T1_soil_microcosm_v2.0.csv. DNA was extracted from soil microcosm samples for microbial community analysis based on 16S rRNA gene sequencing; the resulting operational taxonomic units are presented in T2_soil_OTU_v2.0.csv. Bradyrhizobium sp. strain I71 was isolated from the soil microcosms and acetylene uptake and cell growth data for the isolate over time are shown in T3_soil_isolate_v2.0.csv. Nitrogen fixation assays for the pure culture of Bradyrhizobium sp. strain I71 are reported in T4_N2_fixation_v2.0.csv. Acetylene concentrations and cell densities from acetylenotrophic and heterotrophic growth assays for Bradyrhizobium sp. strain I71 are reported in T5_GrowthCurve_v2.0.csv
In this study, we evaluated the presence of acetylenotrophs in TCE-contaminated soils undergoing bioremediation. These data track the isolation process of Bradyrhizobium sp. strain I71 from the aerobic soil microcosms established from TCE-contaminated soil to the pure culture isolate. Members of the Bradyrhizobium are well-known diazotrophs, so nitrogen fixation assays in the pure isolates were conducted. The isolate was tested for its ability for acetylenotrophic and heterotrophic growth.
Revision 2.0 by Shaun Baesman on September 12, 2022. To review the changes that were made, see “Bradyrhizobium_revision_history_v2.0.txt” in the attached files section.
