Cyanobacteria, Cyanotoxin, Cyanotoxin Synthetase Gene, and other Water-Quality Data Collected from Five River Basins in the North Atlantic Appalachian Region, August through September, 2020

Dates

Publication Date: 2024-04-29
Start Date: 2020-08-31
End Date: 2020-09-09

Citation

Trevino, J.M., Graham, J.L., Foster, G.M., Gifford, S.R., Rosen, J.J., Coles, J.F., Culbertson, C.W., Heckathorn, H.A., Jastram, J.D., Olds, H.T., Schill, W.B., and Stelzer, E.A., 2024, Cyanobacteria, cyanotoxin, cyanotoxin synthetase gene, and other water-quality data collected from five river basins in the North Atlantic Appalachian Region, August through September, 2020: U.S. Geological Survey data release, https://doi.org/10.5066/P94LAHHM.

Summary

This data release contains cyanotoxin, chlorophyll-a, and pheophytin-a concentration, cyanobacterial genetics, phytoplankton community composition, and multiparameter sonde data collected from 20 sites in five northeastern United States river basins (Penobscot (ME), Santuit (MA), York (VA-WV), Salem (NJ), and Peconic (NY)). Solid Phase Adsorption Toxin Tracking (SPATT) passive samplers were deployed at all sites between August 31 and September 2, 2020, and retrieved after 7 days. Discrete water samples were collected when SPATTs were deployed, and at 2 sites (USGS station IDs 01670257, 0167014792), samples were also collected when the SPATTs were recovered. Sonde data were collected when deploying and retrieving the SPATTs. The SPATT [...]

Summary

This data release contains cyanotoxin, chlorophyll-a, and pheophytin-a concentration, cyanobacterial genetics, phytoplankton community composition, and multiparameter sonde data collected from 20 sites in five northeastern United States river basins (Penobscot (ME), Santuit (MA), York (VA-WV), Salem (NJ), and Peconic (NY)). Solid Phase Adsorption Toxin Tracking (SPATT) passive samplers were deployed at all sites between August 31 and September 2, 2020, and retrieved after 7 days. Discrete water samples were collected when SPATTs were deployed, and at 2 sites (USGS station IDs 01670257, 0167014792), samples were also collected when the SPATTs were recovered. Sonde data were collected when deploying and retrieving the SPATTs. The SPATT samples were extracted and analyzed for cyanotoxins (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by enzyme-linked immunosorbent assay (ELISA) and liquid chromatography tandem mass spectrometry (LC/MS/MS). Sonde data included measurements of water temperature, pH, specific conductance, dissolved oxygen, turbidity, chlorophyll, phycocyanin, and phycoerythrin fluorescence (in relative fluorescence units and micrograms per liter), and nitrate, however some sensors were not available at all locations. Discrete samples were analyzed for total cyanotoxin concentration (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by ELISA and LC/MS/MS, chlorophyll-a, pheophytin-a, cyanotoxin synthetase genes, and phytoplankton community composition. Saxitoxin could not being analyzed by LC/MS/MS so an additional ELISA analysis was performed instead.

Contacts

Point of Contact :: Jessica M Trevino
Originator :: Jessica M Trevino, Jennifer L Graham, Guy M Foster, Sabina R Gifford, Joshua J Rosen, James F Coles, Charles W Culbertson, Heather A Heckathorn, John D Jastram, Hayley T Olds, William B Schill, Erin A Stelzer
Metadata Contact :: Jessica M Trevino
Publisher :: U.S. Geological Survey
Distributor :: U.S. Geological Survey - ScienceBase
USGS Mission Area :: Water Resources
SDC Data Owner :: New York Water Science Center

Attached Files

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Metadata_NAARHAB.xml Original FGDC Metadata	View	117.71 KB	application/fgdc+xml
CyanotoxinConc.txt		6.65 KB	text/plain
CyanotoxinGenetics.txt		3.84 KB	text/plain
LabChlorophyllPheophytin.txt		3.05 KB	text/plain
SondeData.txt		6.82 KB	text/plain
SPATTCyanotoxinConc.txt		6.81 KB	text/plain
StationList.txt		2.39 KB	text/plain
CyanobacterialAssayStandardCurve.txt		1.09 KB	text/plain
Phytoplankton.txt		89.68 KB	text/plain

Shapefile: StationLocations.zip
StationLocations.cpg		5 Bytes
StationLocations.dbf		20.83 KB
StationLocations.prj		145 Bytes
StationLocations.sbn		308 Bytes
StationLocations.sbx		124 Bytes
StationLocations.shp		660 Bytes
StationLocations.shp.xml		8 KB
StationLocations.shx		260 Bytes

Related External Resources

Type: Method

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Arar, E. J. AND G. B. COLLINS. Method 445.0 In Vitro Determination of Chlorophyll a and Pheophytin ain Marine and Freshwater Algae by Fluorescence. U.S. Environmental Protection Agency, Washington, DC, 1997.	https://cfpub.epa.gov/si/si_public_record_report.cfm?Lab=NERL&dirEntryId=309417
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ABRAXIS® Cylindrospermopsin ELISA (Microtiter Plate) Enzyme-Linked Immunosorbent Assay for the Determination of Cylindrospermopsin in Water Product No. 522011	https://www.goldstandarddiagnostics.us/media/16556/ug-21-059-rev-02-abraxis-cylindrospermopsin-elisa_522011.pdf
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Type: Citation

Austin, B.J. and B.E. Haggard. 2022. Measurable microcystin in Ozark streams was rare during summer 2018 baseflow conditions. Agriculture and Environmental Letters 7(1): e20069. Available at: https://acsess.onlinelibrary.wiley.com/doi/full/10.1002/ael2.20069	https://doi.org/10.1002/ael2.20069
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Purpose

These data were collected by members of the USGS North Atlantic Appalachian Region Harmful Algal Bloom Capability Team as part of a pilot study to: 1) capture a snapshot of the occurrence of cyanobacteria and associated cyanotoxins in coastal rivers and estuaries throughout the region; 2) further our understanding of the strengths and weaknesses of the use of passive samplers as a supplement to, or in place of, more traditional sample collection methods; and 3) establish a foundational dataset upon which more detailed studies can be developed.

Cyanobacteria, Cyanotoxin, Cyanotoxin Synthetase Gene, and other Water-Quality Data Collected from Five River Basins in the North Atlantic Appalachian Region, August through September, 2020

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