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  3. Development and testing of a qPCR assay for Lampsilis siliquoidea eDNA

Development and testing of a qPCR assay for Lampsilis siliquoidea eDNA

Dates

Publication Date
Start Date
2019-08-23
End Date
2023-03-06

Citation

Klymus, K.E., Ruiz-Ramos, D.V., Schreier, T.M., Merkes, C.M., Thompson, N.L., Voshage, M.C., and Richter, C.A., 2023, Development and testing of a qPCR assay for Lampsilis siliquoidea eDNA: U.S. Geological Survey data release, https://doi.org/10.5066/P9NFR54T.

Summary

Data describe specificity and sensitivity testing for the L.sil2 assay. This assay is used to amplify eDNA from the freshwater mussel Lampsilis siliquoidea.

Contacts

Point of Contact :
Katy E Klymus
Originator :
Katy E Klymus, Dannise V Ruiz Ramos, Theresa M Schreier, Christopher M Merkes, Nathan L Thompson, Megan C Voshage, Cathy A Richter
Metadata Contact :
CERC Data Managers
Publisher :
U.S. Geological Survey
Data Owner :
Columbia Environmental Research Center
SDC Data Owner :
Columbia Environmental Research Center
USGS Mission Area :
Ecosystems
Distributor :
U.S. Geological Survey - ScienceBase

Attached Files

Click on title to download individual files attached to this item.

Metadata_Testing_of_Lsil2_qPCR_assay_for_Lampsilis_siliquoidea_eDNA.xml
Original FGDC Metadata
View 		26.23 KB application/fgdc+xml
Lsil2_Assay_Specificity.csv 3.5 KB text/csv
Lsil2_LOD_LOQ.csv 9.97 KB text/csv

Purpose

Data were collected to optimize the assay used for detection of Lamspsilis siliquoidea eDNA. The assay is being used in laboratory experiments to measure eDNA shedding and degradation rates.

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Communities

  • Columbia Environmental Research Center (CERC)
  • USGS Data Release Products

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9NFR54T

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