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Deep SEARCH eDNA metabarcoding data

Dates

Publication Date
Start Date
2019-04-01
End Date
2023-10-01

Citation

Aunins, A.W., and Morrison, C.L., 2024, Deep SEARCH eDNA metabarcoding data: U.S. Geological Survey data release, https://doi.org/10.5066/P1HYVYFZ.

Summary

The Deep SEARCH Project (Deep sea exploration to advance research on cold corals/canyon/cold seep habitats) included a cruise in April 2019 aboard the NOAA ship Ron Brown to various sites along the Mid-Atlantic North American coast. An ROV was used to collect samples of bottom water at these sites, which were then subjected to eDNA extraction and analyses. This data release describes what samples were collected for eDNA analyses, and how eDNA was extracted, sequenced, and bioinformatically analyzed.

Contacts

Point of Contact :
Aaron W Aunins
Process Contact :
Aaron W Aunins
Originator :
Aaron W Aunins, Cheryl L Morrison
Metadata Contact :
Aaron W Aunins
Publisher :
U.S. Geological Survey
Distributor :
U.S. Geological Survey - ScienceBase
SDC Data Owner :
Eastern Ecological Science Center
USGS Mission Area :
Ecosystems

Attached Files

Click on title to download individual files attached to this item.

18S_unfiltered_table_excel_with_taxonomy.xlsx 5.93 MB application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
cox1_unfiltered_table_excel_with_taxonomy.xlsx 1.99 MB application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
USGS_eDNA_Deepsearch_metadata.xlsx 30.88 KB application/vnd.openxmlformats-officedocument.spreadsheetml.sheet

Purpose

The data consist of eDNA metabarcoding sequence reads of taxa amplified using nuclear 18S rDNA and mitochondrial cox1 metabarcoding markers., and counts of sequences observed in each sample along with a putative taxonomic assignment. Appropriate use of the data is for metabarcoding data analyses and pipelines.

Rights

This work is marked with Creative Commons Zero v1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/).

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Communities

  • Eastern Ecological Science Center

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Provenance

Additional Information

Identifiers

Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P1HYVYFZ

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