Cyanobacteria, Cyanotoxin, Cyanotoxin Synthetase Gene, and other Water-Quality Data Collected from Five River Basins in the North Atlantic Appalachian Region, August through September, 2020

Dates

Publication Date: 2024-04-29
Start Date: 2020-08-31
End Date: 2020-09-09

Citation

Trevino, J.M., Graham, J.L., Foster, G.M., Gifford, S.R., Rosen, J.J., Coles, J.F., Culbertson, C.W., Heckathorn, H.A., Jastram, J.D., Olds, H.T., Schill, W.B., and Stelzer, E.A., 2024, Cyanobacteria, cyanotoxin, cyanotoxin synthetase gene, and other water-quality data collected from five river basins in the North Atlantic Appalachian Region, August through September, 2020: U.S. Geological Survey data release, https://doi.org/10.5066/P94LAHHM.

Summary

This data release contains cyanotoxin, chlorophyll-a, and pheophytin-a concentration, cyanobacterial genetics, phytoplankton community composition, and multiparameter sonde data collected from 20 sites in five northeastern United States river basins (Penobscot (ME), Santuit (MA), York (VA-WV), Salem (NJ), and Peconic (NY)). Solid Phase Adsorption Toxin Tracking (SPATT) passive samplers were deployed at all sites between August 31 and September 2, 2020, and retrieved after 7 days. Discrete water samples were collected when SPATTs were deployed, and at 2 sites (USGS station IDs 01670257, 0167014792), samples were also collected when the SPATTs were recovered. Sonde data were collected when deploying and retrieving the SPATTs. The SPATT [...]

Summary

This data release contains cyanotoxin, chlorophyll-a, and pheophytin-a concentration, cyanobacterial genetics, phytoplankton community composition, and multiparameter sonde data collected from 20 sites in five northeastern United States river basins (Penobscot (ME), Santuit (MA), York (VA-WV), Salem (NJ), and Peconic (NY)). Solid Phase Adsorption Toxin Tracking (SPATT) passive samplers were deployed at all sites between August 31 and September 2, 2020, and retrieved after 7 days. Discrete water samples were collected when SPATTs were deployed, and at 2 sites (USGS station IDs 01670257, 0167014792), samples were also collected when the SPATTs were recovered. Sonde data were collected when deploying and retrieving the SPATTs. The SPATT samples were extracted and analyzed for cyanotoxins (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by enzyme-linked immunosorbent assay (ELISA) and liquid chromatography tandem mass spectrometry (LC/MS/MS). Sonde data included measurements of water temperature, pH, specific conductance, dissolved oxygen, turbidity, chlorophyll, phycocyanin, and phycoerythrin fluorescence (in relative fluorescence units and micrograms per liter), and nitrate, however some sensors were not available at all locations. Discrete samples were analyzed for total cyanotoxin concentration (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by ELISA and LC/MS/MS, chlorophyll-a, pheophytin-a, cyanotoxin synthetase genes, and phytoplankton community composition. Saxitoxin could not being analyzed by LC/MS/MS so an additional ELISA analysis was performed instead.

Contacts

Point of Contact :: Jessica M Trevino
Originator :: Jessica M Trevino, Jennifer L Graham, Guy M Foster, Sabina R Gifford, Joshua J Rosen, James F Coles, Charles W Culbertson, Heather A Heckathorn, John D Jastram, Hayley T Olds, William B Schill, Erin A Stelzer
Metadata Contact :: Jessica M Trevino
Publisher :: U.S. Geological Survey
Distributor :: U.S. Geological Survey - ScienceBase
USGS Mission Area :: Water Resources
SDC Data Owner :: New York Water Science Center

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Metadata_NAARHAB.xml Original FGDC Metadata	View	117.71 KB	application/fgdc+xml
Phytoplankton.txt		89.68 KB	text/plain
CyanobacterialAssayStandardCurve.txt		1.09 KB	text/plain
CyanotoxinGenetics.txt		3.84 KB	text/plain
LabChlorophyllPheophytin.txt		3.05 KB	text/plain
SondeData.txt		6.82 KB	text/plain
SPATTCyanotoxinConc.txt		6.81 KB	text/plain
StationList.txt		2.39 KB	text/plain
CyanotoxinConc.txt		6.65 KB	text/plain

Shapefile: StationLocations.zip
StationLocations.cpg		5 Bytes
StationLocations.dbf		20.83 KB
StationLocations.prj		145 Bytes
StationLocations.sbn		308 Bytes
StationLocations.sbx		124 Bytes
StationLocations.shp		660 Bytes
StationLocations.shp.xml		8 KB
StationLocations.shx		260 Bytes

Related External Resources

Type: Method

Method 546: Determination of Total Microcystins and Nodularins in Drinking Water and Ambient Water by Adda Enzyme-Linked Immunosorbent Assay (2016)	https://www.epa.gov/dwanalyticalmethods/method-546-determination-total-microcystins-and-nodularins-drinking-water-and
10200 PLANKTON Standard Methods For the Examination of Water and Wastewater, 23rd. https://www.standardmethods.org/doi/10.2105/SMWW.2882.207	https://www.standardmethods.org/doi/10.2105/SMWW.2882.207
Arar, E. J. AND G. B. COLLINS. Method 445.0 In Vitro Determination of Chlorophyll a and Pheophytin ain Marine and Freshwater Algae by Fluorescence. U.S. Environmental Protection Agency, Washington, DC, 1997.	https://cfpub.epa.gov/si/si_public_record_report.cfm?Lab=NERL&dirEntryId=309417
Lane Jenny Q. , Roddam C. Meiling , Langlois Gregg W. , Kudela Raphael M. , (2010), Application of Solid Phase Adsorption Toxin Tracking (SPATT) for field detection of the hydrophilic phycotoxins domoic acid and saxitoxin in coastal California, Limnol. Oceanogr. Methods, 8, doi:10.4319/lom.2010.8.0645.	https://doi.org/10.4319/lom.2010.8.0645
Kudela, Raphael M. "Characterization and deployment of Solid Phase Adsorption Toxin Tracking (SPATT) resin for monitoring of microcystins in fresh and saltwater." Harmful algae 11 (2011): 117-125.	https://doi.org/10.1016/j.hal.2011.08.006
ABRAXIS® Microcystins-ADDA SAES ELISA Microtiter Plate Streptavidin Amplified Enhanced Sensitivity Enzyme-Linked Immunosorbent Assay for the Congener-Independent* Determination of Microcystins and Nodularins in Water Samples Product No. 520011SAES	https://www.goldstandarddiagnostics.us/media/15659/ug-21-078-rev-01-abraxis-microcystins-adda-streptavidin-amplified-enhanced-sensitivity-saes-elisa_520011saes.pdf
PhycoTech, Inc. Business and Technical Information	https://www.phycotech.com/Portals/0/PDFs/PhycoTech_INFO_PACKET.pdf
Stelzer, E.A., Loftin, K.A., and Struffolino, Pamela, 2013, Relations between DNA- and RNA-based molecular methods for cyanobacteria and microcystin concentration at Maumee Bay State Park Lakeside Beach, Oregon, Ohio, 2012: U.S. Geological Survey Scientific Investigations Report 2013–5189, 9 p., http://dx.doi.org/10.3133/sir20135189.	https://doi.org/10.3133/sir20135189
Rantala A, Rajaniemi-Wacklin P, Lyra C, Lepistö L, Rintala J, Mankiewicz-Boczek J, Sivonen K2006.Detection of Microcystin-Producing Cyanobacteria in Finnish Lakes with Genus-Specific Microcystin Synthetase Gene E (mcyE) PCR and Associations with Environmental Factors. Appl Environ Microbiol72:.https://doi.org/10.1128/AEM.01058-06	https://doi.org/10.1128/AEM.01058-06
Sabart, Marion, Kristell Crenn, Fanny Perrière, Angélique Abila, Martin Leremboure, Jonathan Colombet, Cyril Jousse, and Delphine Latour. "Co-occurrence of microcystin and anatoxin-a in the freshwater lake Aydat (France): Analytical and molecular approaches during a three-year survey." Harmful algae 48 (2015): 12-20.	https://doi.org/10.1016/j.hal.2015.06.007
Sipari H, Rantala-Ylinen A, Jokela J, Oksanen I, Sivonen K2010.Development of a Chip Assay and Quantitative PCR for Detecting Microcystin Synthetase E Gene Expression . Appl Environ Microbiol76:.https://doi.org/10.1128/AEM.00452-10	https://doi.org/10.1128/AEM.00452-10
Al-Tebrineh, Jamal, Leanne A. Pearson, Serhat A. Yasar, and Brett A. Neilan. "A multiplex qPCR targeting hepato-and neurotoxigenic cyanobacteria of global significance." Harmful Algae 15 (2012): 19-25.	https://doi.org/10.1016/j.hal.2011.11.001
Olson Robert J., Sosik Heidi M., (2007), A submersible imaging-in-flow instrument to analyze nano-and microplankton: Imaging FlowCytobot, Limnol. Oceanogr. Methods, 5, doi:10.4319/lom.2007.5.195.	https://doi.org/10.4319/lom.2007.5.195
ABRAXIS® Saxitoxin in Seawater Sample Analysis	https://www.goldstandarddiagnostics.us/media/16867/tb-21-046-rev-03-abraxis-saxitoxin-in-seawater-analysis-elisa.pdf
ABRAXIS® Microcystins-ADDA ELISA Microtiter Plate Enzyme-Linked Immunosorbent Assay for the Congener-Independent* Determination of Microcystins and Nodularins in Water Samples Product No. 520011	https://www.goldstandarddiagnostics.us/media/15635/ug-21-052-rev-01-abraxis-microcystins-adda-elisa_520011.pdf
ABRAXIS® Saxitoxin (PSP) ELISA Microtiter Plate Enzyme-Linked Immunosorbent Assay for the Determination of Saxitoxin (PSP) in Water and Contaminated Samples Product No. 52255B	https://www.goldstandarddiagnostics.us/media/15661/ug-21-081-rev-03-abraxis-saxitoxin-elisa_52255b.pdf
ABRAXIS® Anatoxin-a ELISA Microtiter Plate Enzyme-Linked Immunosorbent Assay for the Determination of Anatoxin-a* in Water Samples Product No. 520060	https://www.goldstandarddiagnostics.us/media/15640/ug-21-057-rev-03-abraxis-anatoxin-a-elisa_520060.pdf
ABRAXIS® Cylindrospermopsin ELISA (Microtiter Plate) Enzyme-Linked Immunosorbent Assay for the Determination of Cylindrospermopsin in Water Product No. 522011	https://www.goldstandarddiagnostics.us/media/16556/ug-21-059-rev-02-abraxis-cylindrospermopsin-elisa_522011.pdf
LCMS-SOP Determination of Microcystins in Water Samples by High Performance Liquid Chromatography (HPLC) with Single Quadrupole Mass Spectrometry (MS)	https://www.protocols.io/view/lcms-sop-determination-of-microcystins-in-water-sa-x54v9m7wmg3e/v1
Birbeck, Johnna A., Judy A. Westrick, Grace M. O’Neill, Brian Spies, and David C. Szlag. 2019. "Comparative Analysis of Microcystin Prevalence in Michigan Lakes by Online Concentration LC/MS/MS and ELISA" Toxins 11, no. 1: 13. https://doi.org/10.3390/toxins11010013	https://doi.org/10.3390/toxins11010013
Method 545: Determination of Cylindrospermopsin and Anatoxin-a in Drinking Water by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC/ESI-MS/MS)	https://www.epa.gov/sites/default/files/2017-10/documents/epa_815-r-15-009_method_545.pdf

Type: Citation

Austin, B.J. and B.E. Haggard. 2022. Measurable microcystin in Ozark streams was rare during summer 2018 baseflow conditions. Agriculture and Environmental Letters 7(1): e20069. Available at: https://acsess.onlinelibrary.wiley.com/doi/full/10.1002/ael2.20069	https://doi.org/10.1002/ael2.20069
Saxitoxin in Water Samples Quick Reference Guide	https://www.goldstandarddiagnostics.us/media/15850/tb-21-047-rev-01-abraxis-saxitoxin-in-water-quick-reference-guide.pdf
Cyanotoxins in Brackish or Seawater Kit Tolerance Reference Guide	https://www.goldstandarddiagnostics.us/media/15826/tb-21-021-rev01-cyanotoxins-in-brackish-or-seawater-kit-reference-guide.pdf
Cyanotoxins in Water Sample Collection Quick Reference Guide for ELISA Plate Assays	https://www.goldstandarddiagnostics.us/media/15827/tb-21-022-rev-01-cyanotoxins-sample-collection-guide.pdf
Leiker, B.M., Abel, J.R., Graham, J.L., Foster, G.M., King, L.R., Stiles, T.C., and Buley, R.P., 2021, Spatial and temporal variability of nutrients and algae in the Republican River and Milford Lake, Kansas, June through November 2017 and May through November 2018: U.S. Geological Survey Scientific Investigations Report 2020–5135, 53 p., https://doi.org/10.3133/sir20205135.	https://doi.org/10.3133/sir20205135

Purpose

These data were collected by members of the USGS North Atlantic Appalachian Region Harmful Algal Bloom Capability Team as part of a pilot study to: 1) capture a snapshot of the occurrence of cyanobacteria and associated cyanotoxins in coastal rivers and estuaries throughout the region; 2) further our understanding of the strengths and weaknesses of the use of passive samplers as a supplement to, or in place of, more traditional sample collection methods; and 3) establish a foundational dataset upon which more detailed studies can be developed.

Cyanobacteria, Cyanotoxin, Cyanotoxin Synthetase Gene, and other Water-Quality Data Collected from Five River Basins in the North Atlantic Appalachian Region, August through September, 2020

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