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This dataset includes microsatellite genotypes for 44 collections of Habroscelimorpha dorsalis dorsalis and 14 collections of H. d. media distributed from Massachusetts to Florida, United States.
This dataset contains sample information about the origin of the butterflies sequenced, and their corresponding SNP genotype data. These data were used to examine patterns of genomic structure of the Karner blue butterfly from throughout its range. The raw sequence data are archived in the GenBank Bioproject PRJNA995790 at: https://www.ncbi.nlm.nih.gov/bioproject/
Categories: Data;
Tags: Genetics,
Karner blue butterfly,
USGS Science Data Catalog (SDC),
Wildlife Biology,
biota
The Bluestone sculpin (Cottus sp.1) is an undescribed freshwater fish endemic to the New River system in Virginia and West Virginia. The taxonomic status of the Bluestone sculpin is unresolved, and its range often overlaps with congeners, with which it may hybridize. The species is most often found in small, cool streams with gravel and rubble dominated substrates. Juveniles and adults are found most often in runs and riffles of boulder and rubble (Jenkins and Burkhead 1994). Many of the streams presumed to be within the species’ range run along highways and may be vulnerable to non-point source pollution. Treatment plants and agricultural runoff are also potential threats to the species. To address the science...
Biological indicator taxa have long been used for integrative assessments of water quality, particularly benthic invertebrate groups such as arthropods. While standardized protocols have been developed to calculate 'biological index' scores based on the abundances of these taxa, such systems are challenging to implement at large scales due to the sampling effort required, taxonomic expertise needed, and the need for repeated sampling to reliably discriminate sites. Many of the same taxa detected by traditional surveys can also be detected by genetic analysis of environmental DNA (eDNA), potentially allowing for an alternative formulation of biological indexes that might be faster and more economical to produce....
The Grand Calumet River flows through northwestern Indiana and was designated an EPA Area of Concern (AOC) in 1987. There are multiple legacy pollutants in the river including but not limited to heavy metals and PCBs. To investigate potential causes and patterns of eutrophication in the river, water samples were collected for 16S rRNA microbial and eukaryotic 18S rRNA amplicon sequencing during April - September 2021. This data release describes the resulting sequencing data.
Categories: Data;
Tags: Aquatic Biology,
DNA Sequencing,
Environmental Health,
Environmental Health,
Eukaryotic 18S metabarcoding,
This dataset includes microsatellite genotypes for 16 collections of tiger beetles (Ellipsoptera puritana) distributed in Connecticut and Maryland, United States and 44 collections of Habroscelimorpha dorsalis dorsalis and 14 collections of H. d. media distributed from Massachusetts to Florida, United States.
SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021
Wastewater-based epidemiology (WBE) is used to determine the consumption of, or exposure to, chemicals or pathogens in human populations, and is conducted by collecting representative samples of untreated wastewater (influent) to quantify pathogens shed in the population served by the sampled wastewater system. The Centers for Disease Control and Prevention (CDC) and the US Department of Health and Human Services (HHS), in collaboration with the United States Geological Survey (USGS) and other agencies throughout the federal government, initiated the National Wastewater Surveillance System (NWSS) in response to the COVID-19 pandemic to conduct WBE using analytical methods for the collection and analysis of SARS-CoV-2...
This dataset includes microsatellite genotypes for 16 collections of tiger beetles (Ellipsoptera puritana) distributed in Connecticut and Maryland, United States.
The data being released were part of a project funded by the United States Environmental Protection Agency (USEPA). This study sought to identify the planktonic communities (cyanobacteria, eukaryotic algae) potentially contributing to eutrophication within the Grand Calumet River Area of Concern (AOC) in northern Indiana along the southern shore of Lake Michigan. In 2021, triplicate water samples were collected from five locations during three events, 4/19/21 and 4/20/21, 7/7/21, and 9/15/21. Water samples were processed and planktonic communities were determined by a DNA-based technology (algal metabarcoding). Sampling locations: 1. Columbia Avenue, Hammond, IN 2. Lake George drainage ditch, Hammond, IN 3. Indiana...
Categories: Data;
Tags: Aquatic Biology,
DNA sequencing,
East Chicago, Indiana,
Ecology,
Gary, Indiana,
Data were collected to determine the abundance of nitrogen-fixing microorganisms in Cladophora algae growing on rocks, breakwall structures, or submerged dreissenid mussel beds around southern Lake Michigan. Cladophora samples (N=33) were collected between June and September 2015 from three urban areas: (a) Jeorse Park, East Chicago, Indiana, (b) Calumet Beach, Chicago, Illinois, and (c) North Beach, Racine, Wisconsin, and a National Park site, Portage Lakefront, Indiana Dunes National Park, Indiana. Corresponding lake water samples (N=33) were collected approximately 15-20 feet away from submerged algal mats. Genomic DNA was extracted from water and processed algal samples. The abundance of nitrogen-fixing microorganisms...
The FWS considers the Appalachian grizzled skipper (AGS, Pyrgus wyandot) a Species of Concern and is requesting key information on this species for a status review. According to the RFP, FWS has given AGS full species status, however, AGS’s taxonomic status is considered controversial. Some lepidopterists recognize P. wyandot as a full species whereas others consider it a subspecies, P. centaureae wyandot (pers. comm. B. Leppo, PA Natural Heritage Program; in Chazal et al., 2004). Regardless, FWS can still list AGS as threatened or endangered at the species or subspecies level. In this proposal, AGS will be treated as P. c. wyandot for simplicity during genetic analysis (Objective 4). The AGS is a small butterfly...
The Deep SEARCH Project (Deep sea exploration to advance research on cold corals/canyon/cold seep habitats) included a cruise in April 2019 aboard the NOAA ship Ron Brown to various sites along the Mid-Atlantic North American coast. An ROV was used to collect samples of bottom water at these sites, which were then subjected to eDNA extraction and analyses. This data release describes what samples were collected for eDNA analyses, and how eDNA was extracted, sequenced, and bioinformatically analyzed.
Environmental DNA water samples were collected at multiple sites in Great Smoky Mountains National Park in 2016 and 2017 and analyzed with a droplet digital PCR assay to screen for the presence of smoky mountain madtoms. Smoky Mountain madtoms were detected within a section of Abrams Creek, known as the only stream in Great Smoky Mountains National Park that supports this species.
Categories: Data;
Tags: Genetics,
Great Smoky Mountains National Park,
USGS Science Data Catalog (SDC),
biota,
environment,
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