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Carbon dioxide (CO2) is under consideration as a chemical fish deterrent at pinch points in rivers (e.g., inside locks) to reduce unwanted fish passage. This study evaluated the behavioral responses of invasive round goby (Neogobius melanostomus) to dissolved CO2 in laboratory tanks. The goal of this project was to quantify CO2 concentrations that modify round goby behavior. More specifically, avoidance behavior (voluntary response) and loss of equilibrium (involuntary response) were quantified across 5, 15, and 25°C water temperatures. These data can be used to inform future field testing of CO2 as an invasive fish deterrent. Spreadsheets include data that were used to evaluate the efficacy of carbon dioxide (CO2)...
The table provides all fish collected using two different electrofishing methods at Illinois River sites in 2012 and 2013. Length and weights were taken on most species and gender was taken from Silver Carp. Fishes were categorized whether they were netters (caught by nets) or jumpers (jumped in the boat while sampling) and only netters were used in analyses. Large numbers of shad were collected in 2013 and an additional spreadsheet includes abundance data in an aggregated form for those sites. The data is not sensitive/classified and there are no legal restrictions on who may obtain or use the data.
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Invasive species may vary in their seasonal distribution and abundance due to environmental conditions such as precipitation and temperature. Bigheaded carps, which include silver and bighead carp, are one such taxon of invasive species that appear to change habitats seasonally. Seasonal changes in bigheaded carp distribution may occur because of hydrological changes, water temperature changes, and spawning activities. Monitoring this seasonal dispersal and migration is important for management to control the population size and spread of the species. We examined if environmental DNA (eDNA) approaches could detect seasonal changes in the occurrence of DNA in water samples and used these approaches to calculate the...
This dataset includes the behavioral responses to a broadband sound for common carp in an outdoor concrete pond. Broadband sound (0.06 to 10 kHz) has shown potential as an acoustic deterrent for bigheaded carps, but the response of common carp to broadband sound has not been evaluated. Since common carp are ostariophysians, possessing Weberian ossicles similar to bigheaded carps, it is possible that sound can be used as an acoustical deterrent for all three species.
Carbon dioxide has shown promise as a tool to control movements of invasive Asian carps. We evaluated lethal and sublethal responses of juvenile fat mucket (Lampsilis siliquoidea) mussels to carbon dioxide concentrations (43–269 mg/L, mean concentration) that are effective for deterring carp movement. The 28-d LC50 value (lethal concentration to 50% of the mussels) was 87.0 mg/L (95% confidence interval, CI 78.4–95.9) and at 16-d post-exposure was 76.0 mg/L (95% CI 62.9–90.3). A proportional hazards regression model predicted that juveniles could not survive CO2 concentrations >160 mg/L for more than 2 weeks or >100 mg/L CO2 for more than 30 days. Mean daily shell growth was significantly lower for mussels that...
The invasive sea lamprey poses a substantial threat to fish communities in the Great Lakes. Efforts to control sea lamprey populations typically involve treating tributary streams with lampricides on a recurring cycle. Elevated densities of sea lampreys in the aquatic corridor between Lakes Huron and Erie prompted managers to propose a treatment using Bayluscide®—a granular lampricide formulation that targets larval sea lamprey that reside in sediments. However, there was concern over the potential for adverse effects of this treatment on native freshwater mussels—imperiled animals that also reside in sediments. We estimated the risk of mortality and sub-lethal effects among eight species of adult and sub-adult...
Data from this study will be used to extend the current pH/ alkalinity sea lamprey MLC prediction chart used to set 3-trifluoromethyl-4-nitrophenol (TFM) concentrations for treating streams to control sea lamprey populations in the Great Lakes. The current chart predicts levels of TFM required to kill 99.9% of larval sea lampreys in water with alkalinities up to 260 mg/L. Some streams in lower Michigan, however, exceed the current 260 mg/L alkalinity threshold. Treatment managers have requested an extension of the chart to an alkalinity of 300 mg/L for pHs between 8.0 and 8.5. Exposures were conducted with TFM in water at pH 8.0 and 8.5 at alkalinities 260 and 300. Differences in TFM toxicity between the two alkalinities...
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Eggs were collected in the Upper Mississippi main stem (Pool 9 and Pool 11) during the summer of 2013. Using previously published morphological characteristics, eggs were positively identified as belonging to an invasive Asian carp genus. A subsample of these eggs was subsequently analyzed using molecular methods to determine species identity. Genetic identification of a total of 41 eggs was attempted using the cytochrome c oxidase 1 (COI) gene. Due to the preservation technique used (formalin) and resulting DNA degradation, sequences from only 17 individuals could be recovered. In all cases, non-carp cyprinids were identified as the most likely species identity (usually a Notropis spp.). In previously published...
The efficacy and subsurface application of Zequanox®, a commercially prepared spray-dried powder formulation of Pseudomonas fluorescens (strain CL145A), were evaluated for controlling zebra mussels (Dreissena polymorpha) within 27-m2 enclosures in Lake Minnetonka (Deephaven, Minnesota). Five treatments consisting of (1) two whole water column Zequanox applications, (2) two subsurface Zequanox applications, and (3) an untreated control were completed on each of three independent treatment days during September 2014. The two types of samplers used in the study were (1) custom built multi-plate samplers (type 1 samplers), with wood, perforated aluminum, and tile substrates that were placed into Robinson’s Bay in June...
These datasets include data that was used to evaluate the efficacy of carbon dioxide (CO2) injected under-ice as a tool for reducing the overwinter survival of invasive carps.
The objective of this study was to document the vertical avoidance behavior of the tadpole madtom, as a surrogate to the northern madtom, in response to granular Bayluscide® when applied to control or assess larval sea lamprey populations. The data set consists of one hour recordings of the avoidance behavior of tadpole madtoms after exposure to granular Bayluscide. Each trial (replicated 15 times) consisted of three treated (Bayluscide granules) and three control (washed sand only) clear Plexiglas vertical columns (107 cm in height, 30.5 cm in diameter) with a single madtom per column. Video recordings were analyzed for vertical avoidance at 30 second intervals after addition of Bayluscide granules/washed sand...
Alternatives to chemicals for controlling dreissenid mussels are desirable for environmental compatibility, but few alternatives exist. Previous studies have evaluated the use of electrified fields for stunning and/or killing planktonic life stages of dreissenid mussels, however, the available literature on the use of electrified fields to control adult dreissenid mussels is limited. We evaluated the effects of sinusoidal alternating current (AC) and square- wave pulse direct current (PDC) exposure on the survival of zebra mussels at water temperatures of 10, 15, and 22°C. Peak voltage gradients of ~ 17 and 30 Vp/cm in the AC and PDC exposures, respectively, were continuously applied for 24, 48, or 72 h. Peak power...
Recent studies have shown the potential of acoustic deterrents against invasive silver carp (Hypophthalmichthys molitrix). This study examined the phonotaxic response of the bighead carp (H. nobilis) to pure tones (500– 2000 Hz) and playbacks of broadband sound from an underwater recording of a 100 hp outboard motor (0.06–10 kHz) in an outdoor concrete pond (10 × 5 × 1.2 m) at the U.S. Geological Survey Upper Midwest Environmental Science Center in La Crosse, WI. The number of consecutive times the fish reacted to sound from alter- nating locations at each end of the pond was assessed. Bighead carp were relatively indifferent to the pure tones with median consecutive responses ranging from 0 to 2 reactions away...
The spread of Aquatic Invasive Species (AIS) between the Great Lakes and Mississippi River Basin by way of the Chicago Area Waterway System (CAWS) is a pressing concern to resource managers in the Midwest region. Augmenting this spread are watercrafts traveling though the CAWS locks and dams. AIS are able to attach to boat hulls, equipment, or are present in the surrounding water during lock transfers. It has been proposed that chemically treating boats during lock transfers would be an effective way to reduce the spread of AIS. Of a range of treatments identified as candidates to do this, hot water and dissolved ozone ranked high as effective treatments causing the least amount of environmental impact. This study...
The effectiveness of an acoustic barrier to deter the movement of silver carp, Hypophthalmichthys molitrix, and bighead carp, H. noblis, was evaluated. A pond (10 m, x 5 m x 1.2 m) was divided in half by a concrete-block barrier with a channel (1 m across) allowing fish access to each side. Underwater speakers were placed on each side of the barrier opening and an outboard motor noise (broadband sound; 0.06 – 10 kHz) was broadcast to repel carp that approached within 1 m of the channel. Broadband sound was effective at reducing the number of successful crossings in schools of silver carp, bighead carp and a combined school. Repulsion rates were 82.5% (silver carp), 93.7% (bighead carp) and 90.5% (combined). This...
Bighead Carp, Silver Carp and Grass Carp (collectively Asian carp) were introduced to North America in the 1960s and 70s and have established in the lower Mississippi River basin. Previously published evidence for spawning of these species has been limited in the upper Mississippi River to just below Dam 22 (near Saverton, MO). In 2013 and 2014, we sampled ichthyoplankton at 18 locations in the main stem of the upper Mississippi River from Dam 9 through Dam 19 and in four tributary rivers that empty into the Mississippi River (Des Moines, Skunk, Iowa and Wisconsin Rivers). We identified eggs and larvae using morphological techniques and then used genetic tools to confirm species identity. The spawning events we...
Zequanox® is a commercial formulation of the killed bacterium, Pseudomonas fluorescens (strain CL145A) that has USEPA approval for use in open water to kill dreissenid mussels. Previous nontarget studies demonstrated the safety and selectivity of the product, but the database is limited for macroinvertebrate taxa and exposure conditions. We evaluated the safety of the product to two high value macroinvertebrates, the amphipod Gammarus lacustris lacutris, and nymphs of the burrowing mayfly, Hexagenia spp., at the maximum approved concentration (100 mg/L A.I.) and exposure duration (8 h). Survival of animals was measured at the end of 8 h exposure and at 24 h post-exposure and 96 h post-exposure. Additionally, histological...
Many fishes native to the Gila River Basin, Arizona, are on the decline with about 70 percent of the 17 fish species Federally listed as endangered or threatened. The decline has been partly attributed to the introduction of nonnative fishes that are of recreational interest such as catfish and smallmouth bass. Effective management practices are needed to control the nuisance nonnative fishes in Southwestern United States watersheds to prevent further decline of the native species and facilitate their restoration. An effective approach is the use of chemical toxicants to control the nuisance species. One chemical mixture of interest, Supaverm®, a combination of mebendazole and closantel, has been reported to show...
The effects of temperature and concentration on the viscosity, settling, and distribution of Zequanox®, a dead-cell spray-dried powder formulation of Pseudomonas fluorescens (strain CL145A) used for controlling invasive dreissenid mussels (zebra mussel, Dreissena polymorpha, and quagga mussel, Dreissena bugensis), was investigated. Laboratory tests examined viscosity, delivery and sink rates, mixing, settling, and diffusion characteristics at varying temperatures and concentrations to determine optimal target viscosity for subsurface injection of Zequanox. Furthermore, this target viscosity was used to predict the optimal concentrations of Zequanox at three different environmental temperatures that were later injected...
The goal of the study in which the data were collected to determine the non-target effects of the molluscicide Zequanox on the survival and reproductive success of Fathead minnows in simulated open-water applications. The specific objectives were to evaluate effects of 8-h exposure to 50 mg/L and 100 mg/L AI Zequanox on: 1) survival of adult Fathead minnows, 2) egg deposition, 3) hatchability of eggs, and 4) survival and growth of newly hatched fry.


map background search result map search result map Asian carp eggs cannot be distinguished from other cyprinid species on the basis of morphology alone: Supporting Data Seasonal trends in eDNA detection and occupancy of bigheaded carps, Wabash River, IN: Raw Data Asian carp eggs cannot be distinguished from other cyprinid species on the basis of morphology alone: Supporting Data Seasonal trends in eDNA detection and occupancy of bigheaded carps, Wabash River, IN: Raw Data