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Three SAS code files were used to analyze data for this study and they included: 1) code to compare the weight of fish distributed to the tanks, 2) code to compare the survival of fish by treatment groups, and 3) code to analyze the weight and condition of fish at exposure termination.
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
Introduction The code includes separate logistic regression models for the positive control, negative control, and exposed conditions. The proportion of mortalities (number of dead zebra mussels compared to the total number of zebra mussels in the sample) in the containment bags were modeled with a binomial distribution and a logit link function. A scale parameter was added to the model using the random_residual_statement. Required data includes: test temperature voltage waveform (AC or PDC) mean voltag voltage gradient repetition number exposure duration treatment group total number of animals in the replicate number of mortalities in the replicate Outputs include: Means were calculated for: the percent dead, the...
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
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This dataset contains the supplementary data to the dataset entitled "Assessments of the binary mixtures of four toxicants on Zebra Mussel (Dreissena polymorpha) mortality." These data describe the exposure conditions to include water chemistry before, during, and after exposures as well as mussel condition throughout the study. Monitored water chemistry parameters include dissolved oxygen, temperature, and pH. Periodic measurements of alkalinity, total hardness, and specific conductance where taken before each exposure period. Mussel condition was also assessed at the initiation of each exposure. Mussel condition included shell length and tissue dry weight.
Five R Markdown files (.Rmd) were created for the study entitled: Assessments of the binary mixtures of four toxicants on Zebra Mussel (Dreissena polymorpha) mortality. R codes were used to determine toxicant mixture ratios, summarize data, and analyze dose-response relationships in single toxicant and toxicant mixture trials. Files are described in the readme file in the order in which they were used for the study. Each R Markdown file has a complimentary R Script file (.R) with the same file name. All R Markdown files were created in RStudio version 1.2.1335 with R version 3.6.1 and Tidyverse version 1.3.0. Additional packages are listed in braces, “{},” with the corresponding R Markdown description.
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
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This dataset captures the Zebra Mussel dose-response to four independent toxicants and each binary mixture of those toxicants. Toxicants included EarthTec QZ, Clam-Trol CT-2 (syn. Spectrus CT1300), niclosamide, and potassium chloride. Each dose of toxicant was verified with either ICP-OES or UHPLC, depending on analyte, and comparison to a standard curve. Mortality was tabulated for each observation.
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Zequanox® is an EPA-registered biopesticide for controlling populations of dreissenid mussels (zebra and quagga mussels). Zequanox has demonstrated selective toxicity toward dreissenid mussels; however, a recent laboratory study indicated the potential for Zequanox to impact a non-target salmonid species. We assessed the potential exposure risk by observing the avoidance behavior of two representative species of cold-, cool-, and warm-water fish (lake trout, brook trout, lake sturgeon, yellow perch, fathead minnow, and bluegill). Fish were subjected to the maximum allowable concentration of Zequanox per the product label (100 mg/L active ingredient). Naïve juvenile fish (n = 30 per species) were individually observed...
The efficacy and subsurface application of Zequanox®, a commercially prepared spray-dried powder formulation of Pseudomonas fluorescens (strain CL145A), were evaluated for controlling zebra mussels (Dreissena polymorpha) within 27-m2 enclosures in Lake Minnetonka (Deephaven, Minnesota). Five treatments consisting of (1) two whole water column Zequanox applications, (2) two subsurface Zequanox applications, and (3) an untreated control were completed on each of three independent treatment days during September 2014. The two types of samplers used in the study were (1) custom built multi-plate samplers (type 1 samplers), with wood, perforated aluminum, and tile substrates that were placed into Robinson’s Bay in June...
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
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Zebra mussels (Dreissena polymorpha) have continued their spread within inland lakes and rivers in North America despite diligent containment and decontamination efforts by natural resource agencies and other stakeholders. Identification of newly infested waterways with early detection surveillance programs allows for rapid response zebra mussel eradication treatments in some situations. Previous eradication treatments have been conducted during times of variable water temperatures and temperature has been shown to influence the efficacy of molluscicides. Natural resource managers would benefit from knowledge regarding the impacts of water temperature and exposure duration on toxicity of molluscicides to zebra mussels....
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We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
Alternatives to chemicals for controlling dreissenid mussels are desirable for environmental compatibility, but few alternatives exist. Previous studies have evaluated the use of electrified fields for stunning and/or killing planktonic life stages of dreissenid mussels, however, the available literature on the use of electrified fields to control adult dreissenid mussels is limited. We evaluated the effects of sinusoidal alternating current (AC) and square- wave pulse direct current (PDC) exposure on the survival of zebra mussels at water temperatures of 10, 15, and 22°C. Peak voltage gradients of ~ 17 and 30 Vp/cm in the AC and PDC exposures, respectively, were continuously applied for 24, 48, or 72 h. Peak power...
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
Two SAS code files were used to analyze data for this study and they included: 1) code to compare the condition of unique cohorts of zebra mussel test animals at 3 time points (pre-exposure, exposure termination, and post-exposure termination), and 2) code to predict the lethal concentration of test articles required to cause 50 and 99% mortality (LC50 and LC99, respectively) for each toxicant, exposure duration, and temperature combination.
The effects of temperature and concentration on the viscosity, settling, and distribution of Zequanox®, a dead-cell spray-dried powder formulation of Pseudomonas fluorescens (strain CL145A) used for controlling invasive dreissenid mussels (zebra mussel, Dreissena polymorpha, and quagga mussel, Dreissena bugensis), was investigated. Laboratory tests examined viscosity, delivery and sink rates, mixing, settling, and diffusion characteristics at varying temperatures and concentrations to determine optimal target viscosity for subsurface injection of Zequanox. Furthermore, this target viscosity was used to predict the optimal concentrations of Zequanox at three different environmental temperatures that were later injected...
The environmental fate, persistence, and point-source discharge of traditional molluscicidal compounds led to the development of an alternative biomolluscicide, Zequanox. Previous studies evaluated the efficacy and non-target animal safety of Zequanox in laboratory, mesocosm, and field enclosure studies. One study indicated sensitivity of salmonid species and lake sturgeon (Acipenser fulvescens Rafinesque 1817) following exposure to Zequanox, however, the exposures were not conducted in a manner consistent with the product label. This laboratory study evaluated sublethal and lethal impacts of Zequanox on lake sturgeon and lake trout (Salvelinus namaycush Walbaum in Artedi, 1792) following exposures that were conducted...
map background search result map search result map Assessment of uncontained Zequanox applications in a Midwestern lake data Plots - Assessment of uncontained Zequanox applications in a Midwestern lake data Unionid Shape File Info for Assessment of uncontained Zequanox applications in a Midwestern lake Zequanox Concentration - Assessment of uncontained Zequanox applications in a Midwestern lake data Zebra Mussel Density - Assessment of uncontained Zequanox applications in a Midwestern lake data Zebra Mussel Mortality - Assessment of uncontained Zequanox applications in a Midwestern lake data Unionid Shape File Info for Assessment of uncontained Zequanox applications in a Midwestern lake Zequanox Concentration - Assessment of uncontained Zequanox applications in a Midwestern lake data Zebra Mussel Mortality - Assessment of uncontained Zequanox applications in a Midwestern lake data Zebra Mussel Density - Assessment of uncontained Zequanox applications in a Midwestern lake data Plots - Assessment of uncontained Zequanox applications in a Midwestern lake data Assessment of uncontained Zequanox applications in a Midwestern lake data