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This data release includes physical and chemical characteristics of field collected sediment and soil samples in Missouri representing potential sediment/soil that may enter the water column during construction related activities. Three samples were collected, including Spring River sediment, Osage River bank soil and Columbia crushed limestone. The impacts of increased suspended solid level due to the three samples on early-stage freshwater mussels were examined using three freshwater mussel species, including Fatmucket (Lampsilis siliquoidea), Arkansas Brokenray (Lampsilis reeveiana), and Washboard (Megalonaias nervosa). Specifically, toxicity endpoints including survival, biomass, and growth of juveniles were...
Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic environment. Understanding the environmental factors that influence the decay of eDNA and how these factors impact detection probabilities over time and space could have significant implications for eDNA survey design and data interpretation. Here we experimentally explore eDNA decay in waste materials and reproductive cells obtained...
Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.
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Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of longnose darter DNA. Samples were collected from sites on the St. Francis river, Missouri in 2018.
Data consist of genotypes for pallid sturgeon and paddlefish broodstock and artificially-produced larvae at 19 sturgeon microsatellite loci and four paddlefish loci. Microsatellite genotypes allowed confirmation of homozygous sturgeon DNA and absence of paddlefish DNA in haploid gynogens. Data also consist of DNA yield estimates for haploid gynogens for proof of concept that haploid gynogens can provide sufficient DNA quantity for restriction site-associated DNA sequencing studies.
Text files containing data regarding attributes of lake trout egg thiamine concentrations from sites in lakes Huron and Michigan collected during 2019-20, sampling site locations and quality assurance quality control values for comparison to previous years (2017-18) lake trout egg thiamine concentrations.
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Diploid and triploid Grass Carp (Ctenopharyngodon Idella) tagged with acoustic transmitters were observed in Truman Reservoir, Missouri from February 2017 to March 2019 to determine habitat usage in winter. Habitat parameters were measured at used and available sites to model habitat preference. In addition, tagged fish were targeted during removal attempts to determine the efficacy of using tagged Judas Grass Carp in removing wild conspecifics. This dataset includes information on tagged Grass Carp and habitats they selected as well as capture events and characteristics of the wild fish caught during these attempts.
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Survival, behavior, reproduction, morphometric measurements, and tissue analyses of crayfish, mussels, and fish from acute toxicity tests with pyrethroid pesticides. Exposure and recovery water quality data are also included for quality assurance purposes.
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This dataset contains the results of a laboratory chronic bioassay with 6-week-old juvenile mussels (Fatmucket, Lampsilis siliquoidea) in a 28-day zinc exposure (120 and 240 micrograms zinc per liter) with endpoints of survival and growth (biomass and length) following standard ASTM methods. Surviving mussels were transferred from the control and treatment groups into a culture pond and their survival and growth were monitored for 56 days. In this data release we report individual biological, chemical, and water quality measurements from the laboratory toxicity test exposure and pond grow-out period.
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Data were collected in association with the application of two physical barrier treatments, bentonite clay (a sealing agent) and expanding foam (an insulation sealant), in natural pond-burrows to suppress or kill the invasive red swamp crayfish (Procambarus clarkii). Data include burrow diameter and depth, crayfish sex and size, and determination of the treatment reaching the burrow chamber.
This data release includes sampling location data, field-collected wetland attribute data, field-collected water chemistry data, laboratory-processed water chemistry data (anions, cations, alkalinity, nutrients, chlorophyll a concentrations, dissolved organic carbon, and specific ultraviolet absorbance, pesticide concentrations), dry mass of adult aquatic insects emerging from the surface of the wetlands, taxonomic classifications of the insects collected, and whole-body pesticide concentrations for adult aquatic insects emerging from wadeable wetlands in cropland and grassland landscapes across a salinity/hydrology gradient (N = 14 wetlands in 2015, N = 15 wetlands in 2016). Sampling was completed in late spring,...
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Using juvenile individuals of 3 species of carp (Family: Cyprinidae), we tested the potential for six amino acids to elicit an extracellular electro-olfactory response within the naris using gelatin-based electrodes. The amino acids L-alanine, L-arginine, L-aspartic acid, L-asparagine, L-glutamine, and L‑glutamic acid were tested on Grass Carp (Ctenopharyngodon idella), Bighead Carp (Hypophthalmichthys nobilis), and Silver Carp (H. molitrix) to provide a comparison among amino acids both within and among species. Each row in the data set represents exposure to a single amino acid. Each individual fish was exposed to up to four amino acids sequentially in a random order, representing a trial; each individual was...
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This dataset contains the particle starting locations for particle tracking simulations of the Searcys Bend dye release experiment using the “Fluvial Particle” particle tracking model. This file was generated to approximate the location and distribution of dye as observed in the first aerial image captured 3.5 minutes following the dye release. Although the total number of particles simulated is 1,500,000, simulations were run using a parallel version of the Lagrangian particle tracking algorithm distributed across 120 computational processing units (CPUs). Therefore, the file provides the location information for 12,500 particles (1,500,000 / 120 = 12,500). The file was created by georeferencing a line laterally...
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This dataset contains a series of processed acoustic Doppler current profiler (ADCP) measurements collected by boat on the Missouri River at Searcys Bend near Columbia, Missouri on May 5, 2021, during a dye release experiment at the site. The data presented here have been processed with the Velocity Mapping Toolbox (VMT) and combined into a single shapefile using ArcGIS. The dataset was collected to validate a multidimensional hydrodynamic model.
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We investigated whether the length of time fire chemical weathers on a terrestrial substrate before mixing into aquatic environments alters the chemical’s toxicity when encountered by juvenile rainbow trout. We also looked at whether the type of substrate where the chemical is applied affects its toxicity to juvenile rainbow trout after mixing into water. Two fire chemicals (Phos Chek LC95A-R and Phos Chek MVP-Fx) were applied to four substrate media (low organic content soil, high organic content soil, duff, and gravel) and allowed to age on the substrate for 7, 14, 28 or 56 days. At the end of the assigned weathering period, 96-hour assays were conducted by adding water to the dosed substrate and stocking each...
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A total of 236 Black Carp were collected throughout the year from 2017-2019 by incidental captures of commercial fishers and biologists. Capture date, river basin of capture location, and coordinates were recorded for each Black Carp captured. A subsample of 119 Black Carp varying from 429-1268 millimeters total length were compared for the precision of annuli counts by two experienced readers for the ossified two anterior pectoral fin rays, the ossified anterior dorsal fin ray, sectioned vertebrae, and lapilli otoliths for selection of a single structure based on percent exact agreement, coefficient of variation, and age-frequency plots.
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This project contains data and supporting files from hydrodynamic and particle tracking models of the Missouri River at Searcys Bend developed in conjunction with an experimental dye release at the study site on March 5, 2021. The main objectives of this effort were to develop a hydrodynamic model using topographic and flow data collected in the field for subsequent use in a particle tracking model to simulate dye dispersion during the experiment.
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This dataset contains two- and quasi-three-dimensional hydrodynamic model outputs from the Flow and Sediment Transport with Morphologic Evolution of CHannels (FaSTMECH) hydrodynamic model in the open-source binary Visualization Toolkit (VTK) format (https://vtk.org/). The simulations were run at flows in the range of 185-635 cms at increments of 30 cms. This set of flow conditions pertains to the base lateral eddy viscosity scenario referred to as LEVx1 in Call et al., 2023. Files can be opened using the open-source software program Paraview: (https://www.paraview.org/).
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These two datasets contain metrics from hydrodynamic and particle tracking simulations for two sub-reaches of the Upper Missouri River near Wolf Point, MT. The upstream reach is referred to as the “upper” reach while the downstream reach is referred to as the “lower” reach. Simulations were performed for 16 flows in the range of 185-635 cms at increments of 30 cms.


map background search result map search result map Microsatellite genotypes and DNA yield of artificially produced larval pallid sturgeon, Scaphirhynchus albus Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017 Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018 Thiamine concentrations in lake trout eggs collected from the Great Lakes in 2019-20 Adult aquatic insect emergence, insect pesticide concentrations and water chemistry of wetlands in the Prairie Pothole Region, North Dakota, USA, 2015-16 Water quality, habitat, sampling methods and characteristics for grass carp in Truman Reservoir Missouri, 2017-2019 Survival, behavior, reproduction, morphometric measurements, and tissue analyses of crayfish, mussels, and fish from acute pesticide toxicity tests Electro-olfactory responses of Grass Carp, Bighead Carp, and Silver Carp to the amino acids L-alanine, L-arginine, L-aspartic acid, L-asparagine, L-glutamine, and L‑glutamic acid Supporting files for particle tracking simulations of the May 5, 2021 dye release experiment on the Missouri River at Searcys Bend, near Columbia, Missouri Processed acoustic Doppler current profiler (ADCP) measurements of the Missouri River at Searcys Bend near Columbia, Missouri, May 5, 2021 Start location file for the “Fluvial Particle” model used in generating particle tracking simulations of the dye release experiment at Searcys Bend near Columbia, Missouri, May 5, 2021 Survival and growth of the freshwater mussel Lampsilis siliquoidea during a 28-day chronic zinc exposure and subsequent grow-out period Crayfish morphometric measurements, burrow attributes and occupancy in response to physical burrow barriers Alternative complement pathway assay data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture Water chemistry and biological data of Rainbow Trout following aquatic exposure to weathered wildland fire retardants after application to substrate Impact of suspended sediments/soils to juvenile mussels Child 8: Metrics from hydrodynamic and particle tracking simulations of two sub-reaches of the Upper Missouri River near Wolf Point, MT Age estimates of captured Black Carp (Mylopharyngodon piceus) using four calcified structures, 2017-2019 Outputs from hydrodynamic simulations of flows between 185-635 cms at 30 cms increments for the Upper Missouri River near Wolf Point, MT Crayfish morphometric measurements, burrow attributes and occupancy in response to physical burrow barriers Survival, behavior, reproduction, morphometric measurements, and tissue analyses of crayfish, mussels, and fish from acute pesticide toxicity tests Electro-olfactory responses of Grass Carp, Bighead Carp, and Silver Carp to the amino acids L-alanine, L-arginine, L-aspartic acid, L-asparagine, L-glutamine, and L‑glutamic acid Survival and growth of the freshwater mussel Lampsilis siliquoidea during a 28-day chronic zinc exposure and subsequent grow-out period Water chemistry and biological data of Rainbow Trout following aquatic exposure to weathered wildland fire retardants after application to substrate Alternative complement pathway assay data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture Microsatellite genotypes and DNA yield of artificially produced larval pallid sturgeon, Scaphirhynchus albus Adult aquatic insect emergence, insect pesticide concentrations and water chemistry of wetlands in the Prairie Pothole Region, North Dakota, USA, 2015-16 Child 8: Metrics from hydrodynamic and particle tracking simulations of two sub-reaches of the Upper Missouri River near Wolf Point, MT Outputs from hydrodynamic simulations of flows between 185-635 cms at 30 cms increments for the Upper Missouri River near Wolf Point, MT Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018 Water quality, habitat, sampling methods and characteristics for grass carp in Truman Reservoir Missouri, 2017-2019 Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017 Impact of suspended sediments/soils to juvenile mussels Thiamine concentrations in lake trout eggs collected from the Great Lakes in 2019-20 Age estimates of captured Black Carp (Mylopharyngodon piceus) using four calcified structures, 2017-2019