Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, 2019-2022 (ver. 2.0, July 2023)
Dates
Start Date
2019-03-19
End Date
2022-01-05
Publication Date
2023-06-09
Revision
2023-08-29
Citation
Peck, R.W., Munstermann, M., and Atkinson, C., 2023, Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, 2019-2022 (ver. 2.0, July 2023): U.S. Geological Survey data release, https://doi.org/10.5066/P9AEQWX0.
Summary
This data release includes metadata and tabular data that documents lab and field trials testing the efficacy of carbon dioxide (CO2) gas diffused into water to manage invasive fish in anchialine pools. The data release also includes information documenting sensitivity, specificity, and accuracy of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia (Oreochromis mosambicus), western mosquitofish (Gambusia affinis) and guppies (Poecilia reticulata). In total there are 11 datasets, 9 describing lab trials and 2 describing field trials. Lab data include 1) initial water conditions during pilot study, 2) behavioral response of fish to CO2 during pilot study, 3) survival of fish following [...]
Summary
This data release includes metadata and tabular data that documents lab and field trials testing the efficacy of carbon dioxide (CO2) gas diffused into water to manage invasive fish in anchialine pools. The data release also includes information documenting sensitivity, specificity, and accuracy of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia (Oreochromis mosambicus), western mosquitofish (Gambusia affinis) and guppies (Poecilia reticulata). In total there are 11 datasets, 9 describing lab trials and 2 describing field trials. Lab data include 1) initial water conditions during pilot study, 2) behavioral response of fish to CO2 during pilot study, 3) survival of fish following exposure to CO2 during pilot study, 4) initial water conditions during full study, 5) behavioral response of fish to CO2 during full study, 6) survival of fish following exposure to CO2 during full study, 7) specificity of qPCR assays, 8) sensitivity of qPCR assays, and 9) accuracy of qPCR assays for detecting invasive fish from water samples collected from anchialine pools. Field data include 1) pH levels obtained in pools during treatment and 2) body lengths of fish removed during treatment. The lab work was conducted during 2019-2022 at the USGS Kilauea Field Station and field trials were conducted at Puuhonua o Honaunau National Historical Park and Kaloko-Honokohau National Historical Park during 2021-2022. NOTE: This data release was first posted on 06-09-2023, and is available from the author. The current data release (version 2.0) is a revision of that version, and was revised on 07-21-2023.
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CO2 to manage invasive fish, Hawaii Island, 2019-2022_landing page_v2.xml Original FGDC Metadata
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CO2 to manage invasive fish, Hawaii Island, 2019-2022 v2.0.txt “Version History”
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CO2 image for Data Release.jpg “CO2 diffused into anchialine pool to eradicate invasive fish. Photo: Bob Peck. ”
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Related External Resources
Type: Related Primary Publication
Peck, R., Munstermann, M., Hayes, M., Atkinson, C., Beavers, S., Cupp, A., and Banko, P., 2023, Carbon dioxide (CO2) gas and eDNA assessment as tools for eradicating and monitoring invasive fish in anchialine pools in Hawai‘i: Management of Biological Invasions, v. 14, no. 4, p. 749–774, https://doi.org/10.3391/mbi.2023.14.4.11.
Data were collected to document results from pilot and full trials aimed to test the efficacy of CO2 as a tool for eradicating invasive fish from anchialine pools and to evaluate qPCR assays for detecting presence or absence of invasive fish in water samples from anchialine pools. Lab trials identified how reduced pH levels affected the behavior and survival of invasive fish, as well as several native invertebrates that may share habitat with the fish. Lab studies also documented sensitivity, specificity and accuracy of eDNA assays for detecting invasive fish in water samples. Field trials tested CO2 in natural settings at Puuhonua o Honaunau National Historical Park and Koloko-Honokohau National Historical Park. Results from this study are important because they will provide park managers with eDNA monitoring tools and a better understanding of how CO2 can be used to manage invasive fish in anchialine pools.
Preview Image
CO2 diffused into anchialine pool to eradicate invasive fish. Photo: Bob Peck.
Revision 2.0 by Paul Berkowitz on August 29, 2023. To review the changes that were made, see “CO2 to manage invasive fish, Hawaii Island, 2019-2022 v2.0.txt” in the attached files section.