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  4. Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, 2019-2022 (ver. 2.0, July 2023)

Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, 2019-2022 (ver. 2.0, July 2023)

Dates

Start Date
2019-03-19
End Date
2022-01-05
Publication Date
Revision
2023-08-29

Citation

Peck, R.W., Munstermann, M., and Atkinson, C., 2023, Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, 2019-2022 (ver. 2.0, July 2023): U.S. Geological Survey data release, https://doi.org/10.5066/P9AEQWX0.

Summary

This data release includes metadata and tabular data that documents lab and field trials testing the efficacy of carbon dioxide (CO2) gas diffused into water to manage invasive fish in anchialine pools. The data release also includes information documenting sensitivity, specificity, and accuracy of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia (Oreochromis mosambicus), western mosquitofish (Gambusia affinis) and guppies (Poecilia reticulata). In total there are 11 datasets, 9 describing lab trials and 2 describing field trials. Lab data include 1) initial water conditions during pilot study, 2) behavioral response of fish to CO2 during pilot study, 3) survival of fish following [...]

Child Items (11)

Contacts

Point of Contact :
Robert W Peck, Hawaii Cooperative Studies Unit, University of Hawaii at Hilo
Process Contact :
Robert W Peck, Hawaii Cooperative Studies Unit, University of Hawaii at Hilo
Originator :
Robert W Peck, Maya J Munstermann, Carter T Atkinson
Metadata Contact :
PIERC Data Steward
Publisher :
U.S. Geological Survey
SDC Data Owner :
Pacific Island Ecosystems Research Center
USGS Mission Area :
Ecosystems
Distributor :
U.S. Geological Survey - ScienceBase

Attached Files

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CO2 to manage invasive fish, Hawaii Island, 2019-2022_landing page_v2.xml
Original FGDC Metadata
View 		21.72 KB application/fgdc+xml
CO2 to manage invasive fish, Hawaii Island, 2019-2022 v2.0.txt
“Version History” 		1.07 KB text/plain
CO2 image for Data Release.jpg
“CO2 diffused into anchialine pool to eradicate invasive fish. Photo: Bob Peck. ” 		thumbnail 289.57 KB image/jpeg

Purpose

Data were collected to document results from pilot and full trials aimed to test the efficacy of CO2 as a tool for eradicating invasive fish from anchialine pools and to evaluate qPCR assays for detecting presence or absence of invasive fish in water samples from anchialine pools. Lab trials identified how reduced pH levels affected the behavior and survival of invasive fish, as well as several native invertebrates that may share habitat with the fish. Lab studies also documented sensitivity, specificity and accuracy of eDNA assays for detecting invasive fish in water samples. Field trials tested CO2 in natural settings at Puuhonua o Honaunau National Historical Park and Koloko-Honokohau National Historical Park. Results from this study are important because they will provide park managers with eDNA monitoring tools and a better understanding of how CO2 can be used to manage invasive fish in anchialine pools.
CO2 diffused into anchialine pool to eradicate invasive fish. Photo: Bob Peck.
CO2 diffused into anchialine pool to eradicate invasive fish. Photo: Bob Peck.

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Provenance

Revision 2.0 by Paul Berkowitz on August 29, 2023. To review the changes that were made, see “CO2 to manage invasive fish, Hawaii Island, 2019-2022 v2.0.txt” in the attached files section.

Additional Information

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9AEQWX0

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