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We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
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Grass Carp (Ctenopharyngodon idella) are a non-native species to North America that were first introduced for vegetation control in the 1960s. However, wild-reproducing Grass Carp can negatively impact aquatic habitats and aquatic communities by consuming substantial amounts of aquatic vegetation and increasing turbidity. Numerous fisheries techniques have been used in an attempt to control or eradicate Grass Carp, including electrofishing. However, electrofishing efficiency for Grass Carp has been variable, and optimum electrofishing waveforms and parameters for inducing a capture-prone response have not been determined. The objective of this study was to determine the optimum electrofishing waveforms and parameters...
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
The efficacy and subsurface application of Zequanox®, a commercially prepared spray-dried powder formulation of Pseudomonas fluorescens (strain CL145A), were evaluated for controlling zebra mussels (Dreissena polymorpha) within 27-m2 enclosures in Lake Minnetonka (Deephaven, Minnesota). Five treatments consisting of (1) two whole water column Zequanox applications, (2) two subsurface Zequanox applications, and (3) an untreated control were completed on each of three independent treatment days during September 2014. The two types of samplers used in the study were (1) custom built multi-plate samplers (type 1 samplers), with wood, perforated aluminum, and tile substrates that were placed into Robinson’s Bay in June...
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
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Zebra mussels (Dreissena polymorpha) have continued their spread within inland lakes and rivers in North America despite diligent containment and decontamination efforts by natural resource agencies and other stakeholders. Identification of newly infested waterways with early detection surveillance programs allows for rapid response zebra mussel eradication treatments in some situations. Previous eradication treatments have been conducted during times of variable water temperatures and temperature has been shown to influence the efficacy of molluscicides. Natural resource managers would benefit from knowledge regarding the impacts of water temperature and exposure duration on toxicity of molluscicides to zebra mussels....
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We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
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Bayluscide, the 2-aminoethanol salt of niclosamide (2′, 5-dichloro-4′-nitrosalicylanilide), is a pesticide that is used in conjunction with TFM (4-Nitro-3-(trifluoromethyl)phenol) to treat streams tributary to the Great Lakes that are infested with invasive parasitic sea lamprey (Petromyzon marinus). The application TFM with 0.5 to 2.0 percent (%) Bayluscide can significantly reduce the amount of TFM required to achieve effective control. The current formulation of Bayluscide used in combination TFM during stream treatments conducted the Great Lakes Fishery Commission binational Sea Lamprey Control Program is an emulsifiable concentrate (EC). The Bayluscide EC formulation is highly effective; however, it degrades...
Alternatives to chemicals for controlling dreissenid mussels are desirable for environmental compatibility, but few alternatives exist. Previous studies have evaluated the use of electrified fields for stunning and/or killing planktonic life stages of dreissenid mussels, however, the available literature on the use of electrified fields to control adult dreissenid mussels is limited. We evaluated the effects of sinusoidal alternating current (AC) and square- wave pulse direct current (PDC) exposure on the survival of zebra mussels at water temperatures of 10, 15, and 22°C. Peak voltage gradients of ~ 17 and 30 Vp/cm in the AC and PDC exposures, respectively, were continuously applied for 24, 48, or 72 h. Peak power...
Zequanox® is a commercial formulation of the killed bacterium, Pseudomonas fluorescens (strain CL145A) that has USEPA approval for use in open water to kill dreissenid mussels. Previous nontarget studies demonstrated the safety and selectivity of the product, but the database is limited for macroinvertebrate taxa and exposure conditions. We evaluated the safety of the product to two high value macroinvertebrates, the amphipod Gammarus lacustris lacutris, and nymphs of the burrowing mayfly, Hexagenia spp., at the maximum approved concentration (100 mg/L A.I.) and exposure duration (8 h). Survival of animals was measured at the end of 8 h exposure and at 24 h post-exposure and 96 h post-exposure. Additionally, histological...
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY
The effects of temperature and concentration on the viscosity, settling, and distribution of Zequanox®, a dead-cell spray-dried powder formulation of Pseudomonas fluorescens (strain CL145A) used for controlling invasive dreissenid mussels (zebra mussel, Dreissena polymorpha, and quagga mussel, Dreissena bugensis), was investigated. Laboratory tests examined viscosity, delivery and sink rates, mixing, settling, and diffusion characteristics at varying temperatures and concentrations to determine optimal target viscosity for subsurface injection of Zequanox. Furthermore, this target viscosity was used to predict the optimal concentrations of Zequanox at three different environmental temperatures that were later injected...
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The dataset was generated to verify the suitability of the new TFM bar formulation for wide-scale use in the Sea Lamprey Control Program (SLCP). Dataset includes TFM concentrations of three streams (each used twice) where randomized experimental applications of new and old TFM bar formulation dissolution trials were conducted, TFM concentrations of 12 laboratory flume experimental applications of new and old TFM bar formulation dissolution trials, various collected water chemistry parameters, % Active ingredient data from both new and old TFM bar formulation under normal storage and elevated temperature storage conditions, and bar hardness data from both new and old TFM bar formulation under normal storage and elevated...
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Controlling larval sea lamprey (Petromyzon marinus) in Great Lakes tributaries with 4-nitro-3-(trifluoromethyl)phenol (TFM, a pesticide) stream treatments reduces the number of spawning-phase sea lamprey, an invasive species capable of collapsing Great Lakes fisheries. An important component of stream treatments is ensuring that the proper amount of TFM is applied that will effectively control sea lamprey populations and minimize the effects on non-target species. In this study, replicated streamside bioassays were conducted in May, July, and September at six sea lamprey infested rivers in Michigan to determine potential seasonal changes in sensitivity of lamprey to TFM. Larvae (≥ 60 mm) were collected with backpack...
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Zebra mussels (Dreissena polymorpha Pallas 1771) are a tenacious aquatic invasive species in the United States and new infestations can rapidly expand into dense colonies. Zebra mussels were first reported in Marion Lake (Dakota County, Minnesota) in September 2017 and surveys indicated the infestation was likely isolated near the public boat access. A 2.4-hectare area, containing the known zebra mussel infestation, was enclosed and treated for nine days with EarthTec QZ®, a copper-based molluscicide, in an attempt to eradicate the zebra mussels. We conducted an on-site bioassay to confirm treatment efficacy. The bioassay was conducted in mobile assay trailer that received a continuous flow of treated lake water...
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The data for this project can be broken down into three sub-groups: TFM Pellet data, Temp data, and Water Quality data. Each subgroup and associated csv files are explained below: TFM Pellet data: The TFM Pellet dataset consists of 36 individual csv files with each file having a unique name making it representative of 1 trial of the study. For example “0.08 mps at 12C Trials rep 1.csv” would be the first replicate with a flow of 0.08 meters per second and a water temperature of 12°C. Each of the csv files has 16 columns of data. Target Temp: The target temperature of the trial Target Velocity: The target velocity (m/sec) of the trial Replicate #: The rep of the trial Actual Temp: The actual measured temp at the...
The environmental fate, persistence, and point-source discharge of traditional molluscicidal compounds led to the development of an alternative biomolluscicide, Zequanox. Previous studies evaluated the efficacy and non-target animal safety of Zequanox in laboratory, mesocosm, and field enclosure studies. One study indicated sensitivity of salmonid species and lake sturgeon (Acipenser fulvescens Rafinesque 1817) following exposure to Zequanox, however, the exposures were not conducted in a manner consistent with the product label. This laboratory study evaluated sublethal and lethal impacts of Zequanox on lake sturgeon and lake trout (Salvelinus namaycush Walbaum in Artedi, 1792) following exposures that were conducted...
The goal of this study was to complete a comprehensive evaluation of a low-dose copper treatment for zebra mussel (Dreissena polymorpha Pallas 1771) suppression. The treatment objective was to maintain an average copper concentration of 60 µg/L in waters above the thermocline for 10 consecutive days. The study was conducted in Lake Minnetonka (Hennepin County, Minnesota) and the copper treatment was applied to St. Albans Bay (66.3-ha). Robinson Bay (37.2-ha) was used as an untreated control reference site. Five locations were identified as sampling sites throughout each bay and marked with a buoy. Test animals were held, and samples were collected in the vicinity of these sampling buoys before, during, and after...


    map background search result map search result map Bioassay Verification of a Zebra Mussel (Dreissena polymorpha) Eradication Treatment: Data Assessment of uncontained Zequanox applications in a Midwestern lake data Plots - Assessment of uncontained Zequanox applications in a Midwestern lake data Unionid Shape File Info for Assessment of uncontained Zequanox applications in a Midwestern lake Zequanox Concentration - Assessment of uncontained Zequanox applications in a Midwestern lake data Zebra Mussel Density - Assessment of uncontained Zequanox applications in a Midwestern lake data Zebra Mussel Mortality - Assessment of uncontained Zequanox applications in a Midwestern lake data Data Release for Laboratory and field comparisons of TFM bar formulations used to treat small streams for larval sea lamprey Data and Code Release: Determination of Larval Sea Lamprey (Petromyzon marinus) Seasonal Sensitivity Differences to Lampricide Treatment Data and code release—Comparative toxicity of emulsifiable concentrate and suspension concentrate formulations of 2′,5-dichloro-4′-nitrosalicylanilide ethanolamine salt Unionid Shape File Info for Assessment of uncontained Zequanox applications in a Midwestern lake Zequanox Concentration - Assessment of uncontained Zequanox applications in a Midwestern lake data Zebra Mussel Mortality - Assessment of uncontained Zequanox applications in a Midwestern lake data Zebra Mussel Density - Assessment of uncontained Zequanox applications in a Midwestern lake data Plots - Assessment of uncontained Zequanox applications in a Midwestern lake data Assessment of uncontained Zequanox applications in a Midwestern lake data Bioassay Verification of a Zebra Mussel (Dreissena polymorpha) Eradication Treatment: Data Data Release for Laboratory and field comparisons of TFM bar formulations used to treat small streams for larval sea lamprey Data and Code Release: Determination of Larval Sea Lamprey (Petromyzon marinus) Seasonal Sensitivity Differences to Lampricide Treatment Data and code release—Comparative toxicity of emulsifiable concentrate and suspension concentrate formulations of 2′,5-dichloro-4′-nitrosalicylanilide ethanolamine salt